GLP条件下非实验因素对实验用豚鼠生理状态的影响

目的研究GLP条件下非实验因素（包括实验操作、不同笼养方式、对照组因素以及维生素C的添加等）对全身过敏实验中豚鼠质量及生理状况的影响情况，为探讨在GLP条件下如何有效的提高实验动物的质量以及规范过敏性反应的评价提供实验依据。方法实验采用计算机软件进行随机分组，雌雄各半。实验在GLP实验室进行，按要求对豚鼠饲养的环境温度、湿度和噪音进行严格的控制。实验按照SFDA颁布的指导原则推荐的全身过敏实验的程序和方案进行。结果空白对照组和溶剂对照组（生理盐水对照组）在致敏期，体重变化差异明显（P〈0．05）；溶剂对照组与阳性对照组相比较，豚鼠的体重增长在实验的各阶段无明显差异（P〉0．05）；对比托盘笼架饲养，大塑料盒饲养在检疫期更有利于雌性豚鼠体重的增长（P〈0．05）；不添加维生素C组豚鼠在致敏期体重减轻明显（P〈0．05），并出现明显的脱毛症状。结论在GLP条件下，虽然环境条件得到了有效的改善和控制，但是一些容易忽视的非实验因素明显影响了豚鼠的质量及生理状况。

Effects of Non-empirical Factors on Physiological States of Laboratory Guinea Pig under GLP Condition

Objective To investigate the effects of non-empirical factors including experimental operation,different raising style,the various Control Group,and addition of Vitamin C on physiological states of laboratory guinea pigs in GLP condition.Method Guinea pigs were divided into five groups with randomization by the computer software.The experiments were done in the GLP laboratory,where the temperature,humidity and noise were controlled according to the requirements.And the experiments were conducted according to the procedure recommended by the systemic anaphylaxis test in the Principle issued by the SFDA.Results In the allergizing period,the blank group and the dissolvent group had obvious distinction in the changes of body weight(P<0.05),which had no obvious distinction between the dissolvent group and the positive group during the three experimental stages(P>0.05).In comparison of the cage-raising style,the plastic box-raising style benefited the weight-increasing of pig guinea during the quarantine period(P<0.05).The body weight loss obviously in the group lack of Vitamin C compare to the control group(P<0.05).Conclusions Although the circumstance condition was greatly improved under the GLP condition,the non-empirical factors obviously influenced the physiological states of laboratory guinea pig.