大鼠骨髓基质干细胞向神经元样细胞的分化

目的:体外定向诱导大鼠骨髓基质干细胞（BMSCs）向神经元样细胞的分化。方法： 用DMEM培养液冲洗骨髓，收集骨髓细胞接种在培养瓶中体外扩增、纯化。用诱导剂诱导BMSCs分化为神经元样细胞。用免疫组化ABC法鉴定神经丝蛋白（NF-200）、微管相关蛋白（MAP-2）、神经元特异核蛋白（NeuN）、巢蛋白(Nestin) 、胶质纤维酸性蛋白(GFAP)、GAD和ChAT的表达。结果： BMSCs经诱导后，胞体增大，并伸出细长突起，与神经元形态相似。免疫组化显示大部分细胞NF-200，MAP-2，NeuN和Nestin表达阳性，(3±0.8)%的细胞GAD表达阳性，(5±0.3)%的细胞ChAT表达阳性，而GFAP 表达阴性。结论： BMSCs可被诱导分化为神经元样细胞，部分细胞可表达GAD与ChAT。

Differentiation of rat bone marrow stromal cells into neuron like cells

OBJECTIVE: To investigate the differentiation of the rat bone marrow stromal cells (BMSCs) into neuron like cells which express glutamic acid decarboxylase (GAD) and choline acetyltransferase (ChAT). METHODS: BMSCs of rats were flushed out with DMEM from femurs marrow and separated, and then planted in plastic flask. After being expanded and purified, the BMSCs were differentiated into neuron-like cells. Neurofilament (NF-200), microassociate protein-2 (MAP-2), neuron-specific nuclear protein (NeuN), nestin, gial fibrillary acidic protein (GFAP), GAD and ChAT were detected by immunohisitochemistry. RESULTS: The induced BMSCs exhibited neuronal morphological characteristics, and the expressions of NF-200, MAP-2, NeuN and nestin were positive, but the expression of GFAP was negative. About (3 +/- 0.8)% of the induced BMSCs expressed GAD, and (5 +/- 0.3)% expressed ChAT. CONCLUSION: BMSCs can differentiate into neuron like cells, and express GAD and ChAT partially.