| SEN病毒部分基因的克隆及序列测定 |
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| 引用本文： | 詹林盛,孟庆华,贾帅争,孙红琰,杜芝燕,王全立. SEN病毒部分基因的克隆及序列测定[J]. 军事医学科学院院刊, 2001, 25(2): 88-90 |
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| 作者姓名： | 詹林盛 孟庆华 贾帅争 孙红琰 杜芝燕 王全立 |
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| 作者单位： | 1. 军事医学科学院野战输血研究所,
2. 北京佑安医院肝病三科, |
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| 摘 要： | 目的：了解我国人群中是否存在SEN病毒(SENV)感染，并分析SENV国内分离株的部分基因序列，方法：在SENV的保守区设计引物，建立巢式PCR方法检测血清中SENV DNA，并对PCR产物进行克隆，测序，结果，7例非甲－非瘐型肝炎，且输血传播病毒（TTV）阴性患者中，2例SENV阳性，其中一株的序列与意大利SENV－D株（AX－25730）相对应位置核苷酸序列同源性为90％，结论：本研究证初了我国存在SENV感染，建立了检测SENV的PCR方法，并对SENV部分基因进行了克隆及测序，对进一步开展SENV诊断和流行病学调查具有重要意义。
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| 关 键 词： | SEN病毒 肝炎病毒 非甲－非瘐型肝炎 聚合酶链反应 序列排列 |
| 文章编号： | 1000-5501(2001)02-0088-03 |
| 修稿时间： | 2001-03-09 |
Cloning and sequencing of partial gene from SENV isolate in China |
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| ZHAN LinSheng,MENG Qinghua,JIA ShuaiZheng,Sun Hongyan,DU ZhiYan,Wang Quanli. Cloning and sequencing of partial gene from SENV isolate in China[J]. Bulletin of the Academy of Military Medical Sciences, 2001, 25(2): 88-90 |
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| Authors: | ZHAN LinSheng MENG Qinghua JIA ShuaiZheng Sun Hongyan DU ZhiYan Wang Quanli |
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| Affiliation: | ZHAN Lin Sheng 1,MENG Qing Hua 2,JIA Shuai Zheng 1,SUN Hong Yan 1,DU Zhi Yan 1,WANG Quan Li 1 |
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| Abstract: | Objective:To investigate the presence of SENV infection among patients in China,and analyze partial nucleotide sequence of SENV isolated from a patient with non A-G hepatitis.Methods:A nested polymerase chain reaction (PCR) assay with primers from ORF1 of SENV genome was established to detect SENV DNA.The PCR product was cloned and sequenced.Results:SENV DNA was positive in 2 of 7 patients with non A-G hepatitis and TTV negative.Partial gene of a SENV isolate was compared with the corresponding region of SENV isolate(AX025730)from Italy and was found that the nucleotide homology was 90%.Conclusions:The results of this study confirmed the presence of SENV infection in China.The development of a PCR assay for SENV DNA detection and the cloning,sequencing of the SENV isolate have important implication for the diagnosis and epidemiological investigation on SENV infection. [ |
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| Keywords: | SENV hepatitis viruses non A-G hepatitis polymerase chain reaction sequencing alignment |
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