特异性抗内毒素鸡蛋黄抗体的制备

目的：制备特异抗内毒素鸡蛋黄免疫球蛋白（Egg yolk immunoglobulin，IgY），探索防治内毒素血症的新途径。方法：用大肠杆菌J5株、内毒素（Lipopolysaccharide，LPS）及类脂A(Lipid A)作抗原免疫25周龄Roman鸡，改良水溶法提取抗体，进行紫外分光光度计检测、SDS-PAGE及Western blot免疫印迹分析，通过酶联染色反应检测其免疫学活性。结果：大肠杆菌J5株、LPS及Lipid A抗体含量分别为11.4、9.2、9.3mg/mL蛋白黄液，质量分数分别为92.3％、87.13％、90.4％，分子质量为180000u，初步鉴定其对内毒素具有特异性结合作用。结论：用大肠杆菌J5株、LPS及LipidA免疫鸡制备的IgY效价高、特异性强、产量大，将是一种防治内毒素血症的新方法。

Production of specific chicken egg yolk antibody against lipopolysaccharide

ObjectiveTo produce specific chicken egg yolk immunogl ob ulin against lipopolysaccharide(LPS) in order to explore a new way of prevention and cure of endotoximia.MethodsRoman hens , 25 week old ,wer e immunized with Escherichia coli J5 , lipopolysaccharide and lipid A respec tively. By a simple method of water dilution protein purification IgY was separa ted f rom egg yolk . IgY was detected by spectrophotometer and analyzed by SDS PAGE a nd western blot, and its immunologic competence was determined by enzyme labelin g stai ning reaction.Results IgY from Escherichia coli J5 , lipo polysaccharide and lipid A was respectively found 11.4 , 9.2 , 9.3 mg/mL egg yo lk and 92.3% , 87.13% , 90.4% purity and its molecular weight was 180 000 u. conclusionIgY from Escheric hia coli J5 , lipo polysaccharide and lipid A against LPS produced by immunizing Roman hens had hig h titer ,well specificity and large production. It would be a new method of prev ention and cure of endotoximia.