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| 荧光素漏出试验替代整体兔眼刺激试验的初步研究 | |
| 引用本文： | 杨光宇,杨杏芬,杨颖,贺锡雯,熊习昆,谢晓萍,谭小华.荧光素漏出试验替代整体兔眼刺激试验的初步研究[J].中国卫生检验杂志,2007,17(3):435-437,445. |
| 作者姓名： | 杨光宇 杨杏芬 杨颖 贺锡雯 熊习昆 谢晓萍 谭小华 |
| 作者单位： | 1. 广东省疾病预防控制中心,广州,510300;中山大学公共卫生学院,广州,510080 2. 广东省疾病预防控制中心,广州,510300 3. 中国疾病预防控制中心,北京,100000 |
| 基金项目： | 卫生部化妆品卫生标准委员会资助项目 |
| 摘 要： | 目的：探讨荧光素漏出试验（FLT）替代整体动物眼刺激试验（Draize eye irritation test，Draize test）的可能性。方法：用FLT对26种阳性参考物和20种化妆品产品进行检测并分级，化妆品产品同步进行Draizetest，比较分析两种方法受试物刺激性分级结果的等级和分级一致性。结果：对于所检测26种阳性参考物和20种化妆品的刺激性分级，FLT与Draize test之间具有较高的一致性（kappa值分别为0．827和0．571，P均〈0．001）。结论：FLT是一种有效的Draizetest体外替代方法，具有良好的应用价值。 |
| 关 键 词： | 眼刺激试验 替代方法 荧光素漏出试验 |
| 文章编号： | 1004-8685（2007）03-0435-04 |
| 收稿时间： | 2006-11-02 |
| 修稿时间： | 2006-11-02 |
Study on fluorescein leakage test as an alternative method of draize eye irritation test |
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| Yang Guang-yu,Yang Xing-fen,Yang Ying,He Xi-wen,Xiong Xi-kun,Xie Xiao-ping,Tan Xiao-hua.Study on fluorescein leakage test as an alternative method of draize eye irritation test[J].Chinses Journal of Health Laboratory Technology,2007,17(3):435-437,445. | |
| Authors: | Yang Guang-yu Yang Xing-fen Yang Ying He Xi-wen Xiong Xi-kun Xie Xiao-ping Tan Xiao-hua |
| Institution: | 1. Guangdong Center for Disease Control and Prevention, Guangzhou 510300, China; 2. School of Public Health, Sun Yat - sen University, Guangzhou 510080, China; 3. China Center for Disease Control and Prevention, Beijing 100000, China |
| Abstract: | Objective:To study the possibility of the fluorescein leakage test(FLT) as an alternative method for in vivo draize eye irritation test(Draize test).Methods:In this study,26 positive referent chemicals with known eye irritant potentiality and 20 cosmetic products with unknown eye irritant assessment were tested by FLT.Meanwhile,the cosmetics were tested by Draize test.In vivo/in vitro comparisons of the positive chemicals and the cosmetics were conducted according to ranking concordance analysis,respectively.Results:FLT was concordant with draize test in the ocular irritant classification of chemicals and cosmetics(kappa=0.827,P<0.001;kappa=0.571,P=0.002).Conclusion:The FLT is a valuable alternative test of draize test that may be applied in the evaluation of the eye irritation properties of cosmetics and ingredients and even other industrial products. |
| Keywords: | Eye irritation Ahemative method Fluorescein leakage test |
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