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| 一种电化学发光免疫法测定NT-proBNP的实验探讨 | |
| 引用本文： | 王向臣,曲建香,贺长虹. 一种电化学发光免疫法测定NT-proBNP的实验探讨[J]. 医学检验与临床, 2008, 19(3) |
| 作者姓名： | 王向臣 曲建香 贺长虹 |
| 作者单位： | 山东省烟台市烟台山医院,烟台,264001;山东省烟台市烟台山医院,烟台,264001;山东省烟台市烟台山医院,烟台,264001 |
| 摘 要： | 目的对脑利钠肽前体(NT-proBNP)电化学发光法测定的原理及此法测定的精密度、分析的灵敏度、干扰试验等内容做进一步的测定或验证,为临床提供方法学的支持。方法使用Roche公司生产的NT-proBNP试剂盒在Roche MODULAR PPE全自动生化免疫分析仪上测定,以对其正常参考范围、精密度、灵敏度等内容进行测定或验证。结果NT-proBNP的正常参考范围为:<125pg/ml。批内精密度CV(%)分别为0.841、.04、0.541、.12;批间精密度CV(%)为4.063、.503、.21、3.83.测量线性范围为5~35000pg/ml。结论此法测试时间短,准确性好,是目前较理想的方法。 |
| 关 键 词： | 脑利钠肽前体 电化学发光免疫法 全自动生化免疫分析仪 |
Study of a Method for NT-proBNP with electrochemiluminescence immunoassay |
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| Wang Xiangchen,Qu Jianxiang,He Changhong. Study of a Method for NT-proBNP with electrochemiluminescence immunoassay[J]. Medical Laboratory Science and, 2008, 19(3) | |
| Authors: | Wang Xiangchen Qu Jianxiang He Changhong |
| Affiliation: | Wang Xiangchen; Qu Jianxiang; He Changhong; |
| Abstract: | Objective To study the principal,precision,accuracy,interference of the electrochemiluminescence immunoassay method for NT-proBNP and to provide the method supporting for the clinical work.Method The automatic analysis or checking was made on the ROCHE automatic biochemical-immune analyzer MODULAR PPE using the NT-proBNP kits of Roche corporation.the normal reference range,precision,accuracy etc,were calculated.Results the normal reference range of NT-proBNP is <125pg/ml,NT-proBNP`s CVs of intra-assay ar... |
| Keywords: | NT-proBNP electrochemiluminescence immunoassay automatic biochemical-immune analyzer |
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