PCNA、VEGF反义寡核苷酸联合治疗裸鼠肝癌的研究

目的 探讨增殖细胞核抗原（PCNA）反义寡核苷酸和血管内皮生长因子（VEGF）反义寡核苷酸单用或联用对裸鼠人肝癌模型肝癌生长的抑制作用。方法 制作裸鼠皮下肝癌模型24只,分PCNA反义核酸治疗组、VEGF反义核酸治疗组、联合治疗组及对照组,接种后24h之内用PCNA反义核酸和（或）VEGF反义核酸皮下注射进行治疗。观察裸鼠瘤体变化及组织形态学改变。结果 PCNA反义核酸治疗组,VEGF反义核酸治疗组和联合治疗组裸鼠肝癌的生长均受到不同程度的抑制,抑瘤率分别为72．8％、44．9％和87．2％。以联合治疗组的疗效最佳。结论 联合应用PCNA和VEGF2种反义核酸治疗肝癌比应用单种反义核酸有更显著的疗效,2种反义核酸具有协同作用。

Effect of PCNA antisense oligooxynucleotides and VEGF antisense oligoxynucleotides on growth of hepatocellular carcinoma transplanted in nude mice

Objective To study the inhibitory effect of PCNA antisense oligonucleotides or/and VEGF antisense oligonucleotides on growth of human hepatocellular carcinoma in nude mice. Methods Human hepatocellular carcinoma transplanted subcutaneously in nude mice model was estabished, and subsequently was devided into four groups: PCNA ASODN group, VEGF ASODN group, combined group and control group. Defferent treaments were given respectively.The weigh and volume of subcutaneous tumors was measured,and the morphological changes of tumor cells was observed under microscope. Results The growth inhibitory rate in the PCNA ASODN group was 72 8%, 44 9% in the VEGF ASODN group, and 87 2% in the combined group. The combined group which was treated with PCNA ASODN and VEGF ASODN showed more effective inhibitory than the PCNA ASODN group or the VEGF ASODN group respectively. Conclusions The growth of human hepatocellular carcinoma in nude mice can be inhibited by PCNA antisense oligonucleotides and VEGF antisense oligonucleotides respectively. Better inhibitory effect can be obtained if PCNA antisense oligonucleotides and VEGF antisense oligonucleotides are combined in use.