人牙周膜细胞克隆与牙骨质瘤细胞克隆体外钙化的比较

目的：研究瘤性成牙骨质细胞和牙周膜细胞克隆体外不同的生物学特性。方法：对瘤性成牙骨质（CTM）细胞和牙周膜（PDL）细胞进行克隆化培养，分别观察细胞的钙化形态并检测培养液中骨钙素（OC）的含量。结果：其钙化的形式有两种，一种为弥散型钙化，一种为结节状钙化。PDL组结节状钙化为细胞钙化总数的16％，而CTM组结节样钙化为钙化总数的62％（P＜0.01)。两种钙化形式的克隆细胞16d时的OC含量有明显的不同。结论：结节状钙化形式可能是成牙骨质细胞钙化的特有形式之一，这对今后牙周细胞的移植和牙周组织工程的研究有一定的指导意义。

Different biological expression between PDL and cementoblastoma cell clones in vitro

AIM:To identify the different biological expression between periodontal ligament (PDL) cell clone and cementoblastoma (CTM) cell clone in vitro . METHODS:The PDL and CTM cell were cultured and cloned. Using radioimmunlogical technique,the morphological patterns of mineralization were detected, osteocalcin (OC) contents in culture were measured. RESULTS: The results showed that there are two typs of in mineralization colons. One was well defined spherical shaped structure and another showed plaque like deposits. Sixteen percent and 62% percent spherical shaped mineralized clons of total mineralization clons were found in PDL group and CTM group ( P <0.01) respectively. OC content during mineralization between spherical shaped and plaque like mineralized clons was different at 16th day. CONCLUSION: These results suggest that the spherical shaped mineralization was one of unique biological behavior in vitro . It is very important for research of cell transplantation and periodontal tissue engineering.