|
|||||
|
|
| miRNA-29c抑制前列腺癌细胞系LNCaP的增殖和侵袭 | |
| 引用本文： | 邓震,唐亮,矫力,许传亮,张振声,曽钦松,孙颖浩. miRNA-29c抑制前列腺癌细胞系LNCaP的增殖和侵袭[J]. 第二军医大学学报, 2011, 32(6): 607-612. DOI: 10.3724/SP.J.1008.2011.00607 |
| 作者姓名： | 邓震 唐亮 矫力 许传亮 张振声 曽钦松 孙颖浩 |
| 作者单位： | 第二军医大学长海医院泌尿外科,上海,200433;第二军医大学长海医院泌尿外科,上海,200433;第二军医大学长海医院泌尿外科,上海,200433;第二军医大学长海医院泌尿外科,上海,200433;第二军医大学长海医院泌尿外科,上海,200433;第二军医大学长海医院泌尿外科,上海,200433;第二军医大学长海医院泌尿外科,上海,200433 |
| 摘 要： | 目的观察miRNA-29c(miR-29c)对前列腺癌细胞系LNCaP增殖和侵袭能力的影响,探讨其潜在的应用价值。方法采用qRT-PCR观察雄激素依赖性(LNCaP)与雄激素非依赖性(LNCaP-AI)前列腺癌细胞中miR-29c的表达差异;采用miR-29c抑制物(anti-miR-29c)下调LNCaP细胞中miR-29c的表达量,绘制LNCaP细胞生长曲线,利用流式细胞仪测定细胞周期;Transwell侵袭实验分析miR-29c抑制前后LNCaP细胞的体外侵袭能力。结果 qRT-PCR显示LNCaP-AI细胞中miR-29c水平明显低于LNCaP细胞(P<0.05);下调LNCaP细胞中miR-29c的表达量,可明显促进LNCaP细胞体外增殖及侵袭能力(P<0.05)。结论 miR-29c的正常表达有利于抑制LNCaP细胞的体外增殖和侵袭,有望成为前列腺癌生物治疗的潜在靶点。 |
| 关 键 词： | 前列腺肿瘤 miR-29c 细胞增殖 肿瘤浸润 |
| 收稿时间： | 2011-03-08 |
| 修稿时间： | 2011-03-21 |
Inhibitory effect of microRNA-29c on proliferation and invasion of human prostate cancer cell line LNCaP |
|
| DENG Zhen,TANG Liang,JIAO Li,XU Chuan-liang,ZHANG Zhen-sheng,ZENG Qin-song and SUN Ying-hao. Inhibitory effect of microRNA-29c on proliferation and invasion of human prostate cancer cell line LNCaP[J]. Former Academic Journal of Second Military Medical University, 2011, 32(6): 607-612. DOI: 10.3724/SP.J.1008.2011.00607 | |
| Authors: | DENG Zhen TANG Liang JIAO Li XU Chuan-liang ZHANG Zhen-sheng ZENG Qin-song SUN Ying-hao |
| Affiliation: | Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
| Abstract: | Objective To observe the effect of microRNA-29c on the proliferation and invasion abilities of human prostate cancer cell line LNCaP,and to discuss its potential application.Methods The difference in miR-29c expression between ADPC(androgen-dependent prostate cancer) and AIPC(androgen-independent prostate cancer) cell lines was observed by real time RT-PCR.MiR-29c-inhibitor(anti-miR-29c)was used to decrease miR-29c expression in LNCaP cells;then the cell proliferation was examined with CCK-8 assay,the cell ... |
| Keywords: | prostatic neoplasms miR-29c cell proliferation neoplasm invasiveness |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《第二军医大学学报》浏览原始摘要信息 | |
| 点击此处可从《第二军医大学学报》下载全文 | |