小干涉RNA质粒抑制Fn14表达对肝癌细胞增殖的影响

目的观察成纤维细胞生长因子诱导的早期反应蛋白14（Fibroblast growth factor-inducible immediate-early response protein 14、Fn14）表达对肝癌细胞增殖的影响。方法构建PAVU6-Fn14 siRNA干涉质粒并将其转染肝癌细胞株QGY7703，MTT法检测抑制Fn14蛋白表达对肝癌细胞增殖的影响，在培养体系加入肿瘤坏死因子相关弱凋亡诱导因子（Tumor necrosis factor related weak inducer of apoptosls、TWEAK）蛋白后检测肝癌细胞增殖活性。结果成功构建PAVU6-Fn14 siRNA干涉质粒，转化肝癌细胞后肝癌细胞增殖受抑，培养体系加入TWEAK蛋白后肝癌细胞增殖活性仍受抑。结论Fn14蛋白表达降低可抑制肝癌细胞的增殖活性，应用RNA干涉技术抑制Fn14表达对肝癌细胞增殖具抑制作用。

The Effect of siRNA Vector Inhibiting Fn14 Expression on Hepatoma Cell Proliferation

Objective To observe the effect of silence of Fn12 gene expression on human hepatoma cell proliferation.Methods The pAVU6-Fn14 siRNA interference vectors were constructed and transfected into hepatoma cell line QGY7703,MTT method was applied in measuring cell proliferation after TWEAK protein was added into cell culture.Results The pAVU6-Fn14 siRNA interference vectors were successfully constructed;after transfection,the proliferation of hepatoma cell line was inhibited,even after TWEAK proteins were added into cell culture.Conclusion The decrease of Fn14 protein expression can inhibit the proliferation activity of hepatoma cells;the hepatoma cell proliferation can be inhibited through inhibiting Fn14 expression with RNA interference technique.