| 一DXFD52相关人肝细胞cDNA的部分序列分析及其反转录病毒载体的构建 |
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| 引用本文： | 何凤田 谭骏. 一DXFD52相关人肝细胞cDNA的部分序列分析及其反转录病毒载体的构建[J]. 免疫学杂志, 1994, 10(4): 235-239 |
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| 作者姓名： | 何凤田 谭骏 |
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| 作者单位： | 第三军医大学免疫学教研室，第三军医大学分子遗传学教研室 |
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| 摘 要： | 为了寻找和研究新的人类基因ｃＤＮＡ，本实验以Ｔ７ＤＮＡ聚合酶对一ＤＸＦＤ５２相关人肝细胞ｃＤＮＡ（ＤＥ）进行了分段部分测定，并将所测各部分序列分别在ＥＭＢＬ（欧洲分子生物学库）中进行核酸同源性检索，结果在库中没有找到任何具有同源性的人类基因或ＤＮＡ片段。因此，我们初步认为ＤＥ为一新的人类基因ｃＤＮＡ片段。同时为初步探讨ＤＥ的功能，我们还成功地将ＤＥ构建于反转录病毒载体ＰＬＸＳＮ上。
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| 关 键 词： | DXFD52 cDNA 反转录病毒 载体 肝 |
Partial Sequence Analysis and Construction of Recombinant Retroviral Vec-tor of a DXFD_(52)-Related Human Hepatocyte CDNA |
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| He Fengtian,Zhu Xihua,Tan Jun,Liu Xin. Partial Sequence Analysis and Construction of Recombinant Retroviral Vec-tor of a DXFD_(52)-Related Human Hepatocyte CDNA[J]. Immunological Journal, 1994, 10(4): 235-239 |
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| Authors: | He Fengtian Zhu Xihua Tan Jun Liu Xin |
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| Abstract: | In order to discovery and study new cDNA of human gene, a DXFD_(52)-related human hepatocyte cDNA(DE)is sequenced partially with T7 DNA polymerase, Then we search the EMBL(European Molecular Biology Laberato-ry) for homology to the sequenced parts of DE. we can not find any homologous human gene or DNA (or cDNA)frag-ment in the library. So we consider that DE may be a new human gene cDNA fragment. We also insert DE into the retro-viral vector pLXSN successfully.which makes it possible to study the function of the cDNA. |
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| Keywords: | DXFD_(52)-related cDNA DNA sequence analysis Retroviral vector |
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