| 大蒜超氧化物歧化酶的纯化 |
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| 引用本文： | 马安德,李毓琦,何俊,魏素萱. 大蒜超氧化物歧化酶的纯化[J]. 南方医科大学学报, 1994, 0(1) |
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| 作者姓名： | 马安德 李毓琦 何俊 魏素萱 |
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| 作者单位： | 第一军医大学化学教研室，华西医科大学化学部 |
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| 摘 要： | 采用二次丙酮沉淀法和ＳｅｐｈａｄｅｘＧ－１００凝胶柱及ＤＥＡＥ－５２离子交换柱分离工艺纯化了大蒜铜锌超氧化物歧化酶。ＳＤＳ－ＰＡＧＥ电泳测得其分子量为３２９３４ｕ（３３２００Ｄａｌｔｏｎ），产品达电泳纯。该酶在２５８ｎｍ处呈最大吸收，对氰化物、过氧化氢敏感。
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| 关 键 词： | 大蒜；超氧化物歧化酶；纯化 |
Purification of superoxide dismutase from garlic |
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| Ma Ande,Li Yuqi,He Jun,Wei Suxuan. Purification of superoxide dismutase from garlic[J]. Journal of Southern Medical University, 1994, 0(1) |
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| Authors: | Ma Ande Li Yuqi He Jun Wei Suxuan |
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| Affiliation: | Ma Ande,Li Yuqi,He Jun,Wei Suxuan Department of Chemistry |
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| Abstract: | opper-and zinc-containing superoxide dismutase (Cu-Zn-SOD)was purified from garlic by twice acetone precipitation and gel fil-tration(Sephadex G-100)and ion exchange chromatography(DEAE-52 ).The enzyme was of electrophoretic purity. The subunit of the enzyme has a molecular weight of 16600 dalton, as were determined by SDS-PAGE,As shown on ultraviolet spectrum,it has a maximum absorption at 258nm. |
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| Keywords: | superoxide dismutase garlic purification |
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