Tim-3基因rs10053538、rs10515746位点多态性与原因不明复发性流产的关系

目的探讨Tim-3基因启动子区rs10053538、rs10515746位点多态性与原因不明复发性流产的关系。方法收集148例原因不明复发性流产患者为研究对象,以153例正常妊娠者为对照。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法和特异性等位基因聚合酶链反应（AS-PCR）法分别检测rs10053538、rs10515746位点多态性,并取部分PCR产物作测序验证。结果原因不明复发性流产患者rs10053538位点GT＋TT基因型及T等位基因分布频率（10.1%和5.1%）与对照组（11.8%和6.5%）比较差异无统计学意义（χ^2=0.205,P=0.651;χ^2=0.592,P=0.441）。rs10515746位点GT＋TT基因型频率及T等位基因分布频率（6.8%和3.4%）与对照组（3.9%和2.0%）比较差异均无统计学意义（χ^2=1.201,P=0.273;χ^2=1.169,P=0.280）。结论 Tim-3基因启动子rs10053538、rs10515746位点多态性与原因不明复发性流产可能无相关性。

The correlation between polymorphisms of promoter in Tim-3 gene and unexplained recurrent spontaneous abortion

Objective To explore the correlationship between polymorphisms of promoter region（rs10053538,rs10515746）in Tim-3gene and unexplained recurrent spontaneous abortion（URSA）.Methods A casecontrol study was conducted on 148women with URSA and 153women with normal pregnancy.Polymerase chain reaction-restiction fragment length polymorphism（PCR-RFLP）and allele-specific polymerase chain reaction（AS-PCR）were used to determine the polymorphism of rs10053538and rs10515746and some were confirmed by DNA sequencing random.Results There were no significant differences of rs10053538polymorphism between the URSA group and the control group on frequencies of GT plusTT genotype and T allele（10.1%vs 11.8%,χ^2=0.155,P=0.694;5.1%vs 6.5%,χ^2=0.592,P=0.441;respectively）.Also,the differences of rs10515746polymorphism on frequencies of GT plusTT genotype or T allele were found not significant（6.8%vs 3.9%,χ^2=1.201,P=0.273;3.4% vs 2.0%,χ^2=1.169,P=0.280;respectively）.Conclusions The polymorphisms of promoter（rs10053538,rs10515746）in Tim-3gene may not be associated with URSA.