转移相关基因nm23和P53及S100A4在晚期胃癌中的表达及与侵袭转移的相关性研究

目的探讨转移相关蛋白（nm23和P53及S100A4）在晚期胃癌转移发展过程中的意义。方法应用组织芯片和免疫组织化学方法研究nm23、P53和S100A4在74例胃癌患者的癌、癌旁、淋巴结转移、远处转移（肝脏、胰腺、卵巢等）组织中的表达。结果同癌旁组织相比，癌组织中P53和S100A4表达明显升高（P〈0．01），而nm23的表达明显降低（P〈0．05）；同癌组织相比，淋巴结中nm23的表达明显降低（P〈0．05）。远处转移组织S100A4表达明显升高（P〈0．01）。3者的阳性表达与胃癌的部分恶性生物学行为有关。癌组织中nm23^＋／P53^＋、P53^＋／S100A4^＋和nm23^＋／S100A4^＋的基因表型比例分别为48例（64．9％）、50例（67．6％）和39例（52．7％），其中P53^＋／S100A4^＋、nm23^＋／S100A4^＋和nm23^＋／P53^＋／S100A4^＋均与胃癌的高转移潜能相关。结论nm23和S100A4在肿瘤转移中发挥了重要作用；联合检测nm23、P53和S100A4的表达可用于评定胃癌的转移潜能。

Expressions of nm23, P53 and S100A4 proteins and their relationships with metastasis potential in gastric carcinoma

Objective To investigate the expression levels of nm23,P53,and S100A4 in gastric carcinoma and their relationships with clinicopathologic parameters and metastasis potential. Methods Pathological specimens from gastric carcinoma,matched para-tumor tissues,metastatic lymph node and distant metastatic tissues were examined for the expression levels of nm23,P53,and S100A4 proteins by tissue microarray technique and immunohistochemistry. Results The expression levels of P53 and S100A4 were upregulated(P< 0.01),while the expression of nm23 downregulated(P< 0.05) in gastric carcinoma compared with non-tumor tissues. S100A4 expression was significantly higher in distant metastatic tissues,while nm23 lower in metastatic lymph nodes than those in cancer tissues. Upregulating expression levels of nm23,P53,and S100A4 were significantly correlated with some malignant behaviour of gastric cancer. The expression rates of nm23+/P53+,P53+/S100A4+,and nm23+/S100A4+immunohistochemical phenotypes were 48/74(64.9%), 50/74(67.6%),and 39/74(52.7%). P53+/S100A4+,nm23+/S100A4+,and nm23+/P53+/S100A4+phenotypes were associated with high metastasis potential of gastric cancer. Conclusions Alteration of nm23,P53,and S100A4 expression may contribute to the development of gastric carcinoma. Nm23 and S100A4 proteins play a critical role in tumor metastasis. Co-detection of the expression of P53,nm23,and/or S100A4 can be used to evaluate high metastasis potential of gastric cancer.