鬼针草总黄酮治疗血吸虫病肝纤维化的实验研究

目的:观察鬼针草总黄酮(total flavones of Bi-dens pilosa L,TFB)对血吸虫病肝纤维化的治疗作用。方法:将小鼠随机分为6组:正常对照组,模型组,治疗组(TFB57.5、115、230mg/kg)和秋水仙碱0.15mg/kg阳性药对照组(n=10)。除正常对照组外,其余各组每只小鼠经腹部皮肤感染日本血吸虫尾蚴诱导肝纤维化模型,并于感染6周后灌胃(i.g.)相应的受试药物,正常组和模型组小鼠灌胃等容量生理盐水,持续治疗8周。实验结束后,取小鼠血,测血清中ALT、AST、总蛋白(TP)及白蛋白(ALB)含量;取小鼠肝脾称重,计算肝、脾指数,并观察小鼠肝脏形态;同时取部分肝组织进行虫卵计数;另取固定部位肝组织作病理组织学检查。结果:TFB57.5mg/kg显著降低血吸虫病肝纤维化小鼠的肝、脾指数;TFB115、230mg/kg显著降低小鼠血清中ALT、AST、ALB含量;明显改善肝脏大体形态。病理组织学检测结果显示TFB115、230mg/kg明显改善血吸虫病肝纤维化小鼠肝组织结构,减轻肝纤维化程度,降低血吸虫肝纤维化小鼠病理评分。结论:TFB对血吸虫病肝纤维化有明显治疗作用。

Therapeutic effects of total flavones of bidens pilosa L on hepatic fibrosis in murine schistomiasis

AIM:To observe the therapeutic effect of total flavones bidens pilosa L(TFB) on experimental schistosoma japonicum hepatic fibrosis in mice.METHODS:50 mice infected with schistosma japonicum cercariae percutanoeusly were divided into 5 groups:model group,TFB 57.5,115,230 mg/kg group and Colchicines 0.15 mg/kg group and other 10 normal mice were used as control group.After treatment with TFB for 8 weeks,the spleen and liver were removed and weighed.The contents of ALT,AST,albumin(ALB)and total proteins(TP) in serum were assayed using the corresponding kits.Moreover,histopathological changes in hepatic tissues were examined and the degree of liver fibrosis was scored.RESULTS:The mice that infected with schistosoma japonicum,had a featuring increment in spleen and liver weights,serum ALT,AST and EPG contents,decrease in serum ALB and TP contents.TFB(230 mg/kg) treatment significantly reduced serum ALT,AST and EPG contents(P<0.01).In addition the hepatic histopathology was also improved in TFB treatment group(115,230 mg/kg)(P<0.01).CONCLUSION:TFB has significant therapeutic effect on schistosoma japonicum-induced liver fibrosis in mice.