外周血CD3+CD45+T细胞在原发性肝癌患者中的表达及意义

目的探讨原发性肝癌（Hepatocellular Carcinoma，HCC）患者外周血中CD3^＋CD56^＋T细胞、CD3^＋CD56^-T细胞、CD3^＋CD56^＋NK细胞在（HCC）细胞免疫中所发挥的作用。方法采用流式细胞技术对14例HCC患者及30例健康对照人群外周血中的CD3^＋CD56^＋T细胞、CD3^＋CD56^-T细胞、CD3^-CD56^＋NK细胞进行检测。结果14例HCC患者与30例正常对照组外周血测定结果比较发现：HCC患者外周血中CD3^＋CD56^-明显降低，CD3^＋CD56^＋则明显升高，与正常对照组比较差异具有极显著性（P均小于0．005）。HCC患者CD3^-CD56^＋（NK）较正常对照组下降，两组比较差异具有显著性（P小于0．05）。结论CD3^＋CD56^-T细胞在HCC患者外周血中增生活跃，提示高表达的CD3^＋CD56^-T细胞在肝癌免疫应答中具有重要意义。

Clinical Significance and Expression of CD3+ CD56+ T Cells in the Peripheral Blood of Patients Suffering From Hepatocellular Carcinoma

Objective To study CD3~+CD56~+T,CD3~+CD56~-T and CD3~-CD56+NK cells in the peripheral blood of patients suffering from hepatocellular carcinoma(HCC) and their roles played in cellular immune responses.Methods Flow cytometry was applied to the test of CD3~+CD56~+T,CD3+CD56~-T and CD3~-CD56~+T cells in the peripheral blood of both 14 cases of HCC and 30 healthy controls.Results A contrastive study of the test results of the peripheral blood in 14 HCC patients and 30 healthy controls indicated that in the peripheral blood of HCC patients CD3~+CD56~-was obviously decreased while CD3~+CD56~+ was obviously increased,and the difference was significant(P<0.05).CD3~-CD56+(NK) in HCC patients was reduced in contrast with that of the control group again with significant difference(P<0.05).Conclusion The active growth of CD3~+CD56~+T in the peripheral blood of HCC patients indicates that the highly expressive CD3~+CD56~+T cells are of great significance in the immune response of hepatocellular carcinoma.