食管鳞状细胞癌CyclinD1的表达及其与CyclinE的相关性研究

目的观察CyclinD1在食管鳞状细胞癌和癌周正常食管组织的表达,从量化角度阐明其在食管鳞状细胞癌组织中表达的病理学意义及其与CyclinE表达的关系。方法用免疫组化MaxVision法检测CyclinD1、CyclinE在食管鳞状细胞癌和癌周正常食管组织的表达,Image-proplus图像分析软件对其表达强度进行定量分析,并用表达的阳性单位（positiveunit,PU）反映其表达强度。结果 CyclinD1蛋白在食管鳞状细胞癌癌细胞核中表达的PU值高于正常食管组织（P〈0.001）;CyclinD1蛋白表达的PU值与食管鳞状细胞癌分化程度有关（P〈0.001）;浸润浆膜层组的CyclinD1蛋白表达的PU值高于无浆膜层浸润组（P=0.037）;伴淋巴结转移组的CyclinD1蛋白表达的PU值高于无淋巴结转移组（P=0.012）;CyclinD1与CyclinE蛋白在食管鳞状细胞癌中表达呈正相关（r=0.650,P〈0.01）。结论 CyclinD1在食管鳞状细胞癌中表达上调,且与生物学特性相关,可作为判断肿瘤患者预后不良的标志之一;CyclinD1和CyclinE可能在食管癌癌变过程中起协同作用。

Expression of CyclinD1in Esophagus Squamous Carcinoma and Its Correlation with CyclinE

Objective To observe the expression of CyclinD1and its correlation with the expression of CyclinE in esophagus squamous carcinoma.Methods The expressions of CyclinD1and CyclinE protein were detected by immunohistochemistry（MaxVision method）using the monoclonal antibody and their intensities were assessed quantitatively by Image-pro plus image analysis system.Results The monoclonal antibody was detected in specific cell nucleus.The positive unit（PU）value of CyclinD1was larger in the esophagus squamous carcinoma than that in the normal esophagus tissue with a statistically significant difference（P0.001）.The expression of CyclinD1was correlated with differentiation of esophagus squamous carcinoma（P0.001）and infiltration degree of esophagus squamous carcinoma（P=0.037）.The PU value of CyclinD1of the nuclei was larger in esophagus squamous carcinoma with lymph node metastasis than those without lymph node metastasis（P=0.012）.There was a positive correlation between CyclinD1protein and CyclinE protein（r=0.650,P0.01）.Conclusions The positive expression of CyclinD1is up-regulated in the esophagus squamous carcinoma.CyclinD1protein relates to the clinicopathological characteristics and prognostic value in esophagus squamous carcinoma.CyclinD1expression is strongly associated with CyclinE expression in esophagus squamous carcinoma.