| The Construction fo Hybrid Plasmid pBR322:RSF1010 |
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| 作者姓名： | 温肇荣 |
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| 作者单位： | Laboratory of |
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| 摘 要： | The plasmids pBR322 and RSF1010,extracted from E.coli HB802 and E.coliC600 respectively,were treated with restrictive endonuclease EcoRI andligated with T4 DNA Iigase.C600 strains were then transformed with thehybrid plasmid(pSMMl),and thus253transformants were obtained.Thefigures of gel electrophoresis and electromicrograph proved that pSMMlwas a pBR322:RSF1010 composite hybrid.In E.coli C600 pSMMl obtainedresistance against ampieilline(Ap),tetracycline(Tc)and streptomycin(Sm).The orientation of pBR322 and RSF1010 in hybrid pSMMl and the failureof introduction of this hybrid into P.putida AC10 were discussed.
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The Construction fo Hybrid Plasmid pBR322:RSF1010 |
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| Wen Zhaorong Laboratory of Molecular Genetics,Second Military Medical College,Shanghai.The Construction fo Hybrid Plasmid pBR322:RSF1010[J].Journal of Medical Colleges of PLA(China),1986(1). |
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| Authors: | Wen Zhaorong Laboratory of Molecular Genetics Second Military Medical College Shanghai |
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| Abstract: | The plasmids pBR322 and RSF1010,extracted from E.coli HB802 and E.coli C600 respectively,were treated with restrictive endonuclease EcoRI and ligated with T4 DNA Iigase.C600 strains were then transformed with the hybrid plasmid(pSMMl),and thus253transformants were obtained.The figures of gel electrophoresis and electromicrograph proved that pSMMl was a pBR322:RSF1010 composite hybrid.In E.coli C600 pSMMl obtained resistance against ampieilline(Ap),tetracycline(Tc)and streptomycin(Sm). The orientation of pBR322 and RSF1010 in hybrid pSMMl and the failure of introduction of this hybrid into P.putida AC10 were discussed. |
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| Keywords: | plasmids cloning vector transformation genetic recombination genetic |
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