维甲酸对肺鳞癌细胞株增殖分化的影响

用 5× 10 -6mol·L-1浓度的全反式维甲酸 (ATRA)作用于人肺鳞癌细胞株 (TUL细胞 ) ,检测细胞生长曲线 ;用MTT法检测细胞生长抑制率 ;用3H TdR掺入法测细胞DNA合成情况 ;透射电镜下观察细胞超微结构改变 ;并观察裸鼠成瘤情况。结果显示 :与对照组比较 ,经ATRA作用后 ,TUL细胞生长速度减慢 ,细胞生长曲线平坦 ,细胞生长抑制率为 42 3 % ,3H TdR掺入率明显减低 (P <0 .0 1) ,细胞DNA合成减少 ,透射电镜观察发现 ,TUL细胞呈良性分化 ,TUL细胞致瘤性明显降低。提示ATRA能抑制TUL细胞增殖 ,诱导分化 ,降低其致瘤性

Effects of retinoic acid on proliferation and differentiation of human lung squamous carcinoma cell line

Human lung squamous carcinoma cell line(TUL cell) was treated with 5×10 -6 ?mol·L -1 all trans retinoic acid(ATRA). Cell numbers were estimated by 0.4% trypon blue stain. MTT test was used for determining the percentage of cell growth inhibition(GI). DNA syntheses of cells were evaluated by 3H thymidine incorporation. Cell ultrastructure was examined by transmission electron microscope(TEM). TUL cells were treated with or without ATRA, and transplanted in cutaneous of Balb/c nude mice. The results showed that: ① The speed of cell growth in study group was smaller than that in control group. ② The GI of cell lines TUL was 42.3%. ③ The percentage of 3H thymidine incorporation of TUL cells was declined significantly. ④ Ultrastructure under TME showed that TUL cells treated with ATRA was suppressed. These results suggest that ATRA has the effects on growth inhibition and differentiation induction of TUL cell.