Involvement of antioxidant enzymes in multiple-drug resistance in a human T-lymphoblastic leukemia-cell line which over-expresses p-glycoprotein |
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| Authors: | Yang M Jiang X Blake D Zhang Z Macey M Newland A Morris C |
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| Affiliation: | UNIV LONDON LONDON HOSP,COLL MED,INFLAMMAT RES GRP,ARTHRIT & RHEUMATISM COUNCIL BLDG,LONDON E1 2AD,ENGLAND. UNIV LONDON LONDON HOSP,COLL MED,DEPT HAEMATOL,LONDON E1 2AD,ENGLAND. |
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| Abstract: | We have compared the specific activities of Cu/Zn superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in a vinblastine sensitive human T-lymphoblastic cell line (CCRF-CEM) and its multiple drug resistant (MDR) counterpart cell line (CEM/VLB100), which over-expresses P-glycoprotein (PGP). We have found that the specific activity Cu/Zn SOD was consistently 38% increased in CEM/VLB100 cells compared with CCRF-CEM cells. In contrast, the activities of CAT and GSH-Px were similar in the two cell lines. These results suggest that MDR in CEM/VLB100 is a complicated phenotype which not only involves a PGP mechanism, but also a SOD protection mechanism against drug-mediated O2.- cytotoxicity. |
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