过氧化氢对人血管内皮细胞表面黏附分子的影响

目的观察过氧化氢(H2O2)对人血管内皮细胞表面黏附分子(ICAM-1)表达的影响。方法采用人源血管内皮细胞株,用低、中、高浓度H2O2刺激内皮细胞24 h,TRIZOL法提取细胞总RNA,采用半定量RT-PCR技术测定各组细胞中ICAM-1的表达,用紫外线凝胶成像分析系统对电泳图像进行吸光度扫描,以待测终产物与内参照吸光度的比值表示其相对含量。结果不同浓度H2O2刺激血管内皮细胞,其ICAM-1表达较正常组明显提高,且中浓度诱导在一定范围内可能已经达到了最高值。结论H2O2能引起ICAM-1的表达增强,从而推测H2O2可能通过刺激ICAM-1的表达而引起动脉粥样硬化。

Influence of Hydrogen Peroxide on human vascular endothelial cell surface adhesion molecule

Objective It is to observe the influence of Hydrogen Peroxide(H2O2) on human vascular endothelial cell surface adhesion molecule(ICAM-1) expression. Methods The endothelial cells were stimulated for 24 h with H2O2 of low,middle and high concentration.The cell total RNA was extracted with TRIZOL method.The expression of ICAM-1 was examined with RT-PCR.The electrophoresis image was scanned absorbance with ultraviolet ray gelatum imaging analytical system and can culate the opposive quantity of ICAM-1 mRNA.The relative amount was expressed with the ratio of waiting determination end product and ento-reference absorbance.Results After the endothelial cells were stimulated by different concentration H2O2,the ICAM-1 expression was obviously higher than that in control group,and the middle concentration inducing might be achieved the highest value at definite extent.Conclusion H2O2 can increase the expression of ICAM-1,so H2O2 may cause atherosclerosis through stimulating the expression of ICAM-1.