HPLC同时测定千金子不同部位中4种有效成分的含量

目的:考察不同产地千金子不同药用部位中4种有效成分的含量差异,为千金子药材质量控制提供依据.方法:采用HPLC,色谱柱为Apollo C18柱(4.6 mm× 250 mm,5μm),流动相乙腈-0.1％磷酸水溶液,梯度洗脱,检测波长280nm,柱温室温.结果:秦皮乙素、千金子素L1、千金子素L2和千金子素L3分别在3.23 ～ 162 mg·L-1(r =0.9998),3.02～152 mg·L-1(r =1.000 0),1.84 ～91.8 mg·L-1(r =0.999 9)和3.64 ～ 182 mg·L-1(r=1.000 0)线性关系良好；平均加样回收率分别为98.2％ (RSD 1.9％),99.8％ (RSD 2.3％),100.9％ (RSD 1.9％),101.9％ (RSD 2.5％).结论:该方法简便、可靠,重复性好,结果准确,可用于比较不同产地千金子不同药用部位的质量差异.

HPLC Simultaneous Determination of Four Effective Components in Different Parts of Seeds of Euphorbia lathyris

Objective:To investigate the content differences of four effective components in different parts of seeds of Euphorbia lathyris in different areas and to be used for total quality control of seeds of E.lathyris.Method:An RP-HPLC method had been developed for detecting.The system consisting of an Apollo C18 column(4.6 mm×250 mm,5 μm) and a mixture of acetonitrile and 0.1% phosphate acid as the mobile phase in gradient mode was adopted.The detection wavelength was set at 280 nm and the column temperature was room temperature.Result:The linear response range was 3.23-162 mg·L-1(r =0.999 8) for aesculetin,3.02-152 mg·L-1(r=1.000 0) for Euphorbia factor L1,1.84-91.8 mg·L-1(r=0.999 9) for Euphorbia factor L2 and 3.64-182 mg·L-1(r =1.000 0) for Euphorbia factor L3.The average recoveries of these components were 98.2%(RSD 1.9%),99.8%(RSD 2.3%),100.9%(RSD 1.9%) and 101.9%(RSD 2.5%),respectively.Conclusion:The method is simple,accurate and reproducible for quality control of seeds of E.lathyris and can be used for comparing the four effective components of seeds of E.lathyris of different parts in different areas.