一测多评法比较不同黄连炮制品中4种生物碱的含量

目的:比较黄连及其不同炮制品中表小檗碱、黄连碱、巴马汀、小檗碱的含量.方法:应用一测多评法同时测定黄连不同炮制品中4种生物碱的含量.Agilent TC-C18色谱柱(2)(4.6 mm ×250 mm,5μm),流动相乙腈-0.05 mol·L-1磷酸二氢钾溶液(50∶50,每100 mL中加十二烷基硫酸钠0.4g,再以磷酸调节pH 4.0),检测波长345 nm,柱温25℃,流速1mL·min-1,理论塔板数以盐酸小檗碱计算应大于2 000.结果:黄连经不同方法炮制后表小檗碱、黄连碱、巴马汀、小檗碱的含量均有不同程度的降低.结论:试验建立的含量测定方法快捷、经济,可作为黄连不同炮制品的质量检测方法.

Comparison of Contents of Four Alkaloids in Different Processed of Coptis chinensis with Quantitative Analysis Multi-components by Single Marker Method

Objective:To compare the contents of epiberberine,coptisine,berberine and palmatine in different processed of Coptis chinensis.Method:Quantitative analysis multi-components by single marker was used to determine the the contents of four alkaloids in different processed C.chinensis.Chromatographic conditions were:Agilent TC-C18 column(2)(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.05 mol · L-1 potassium dihydrogen phosphate(50 ∶ 50,plus 0.4 g sodium dodecyl sulfate per 100 mL,phosphate-regulating pH 4.0),detection wavelength 345 nm,column temperature 25 ℃,flow rate 1 mL · min-1,number of theoretical plates calculated to berberine hydrochloride should be greater than 2 000.Result:The contents of epiberberine,coptisine,berberine and palmatine in different processed of C.chinensis had different degrees of reduction.Conclusion:Established determination method in this test was rapid and economical,it could be used as quality testing method for different processed of C.chinensis.