骨碎补总黄酮对颅骨缺损修复大鼠血清碱性磷酸酶、钙、磷的影响

目的:探讨中药骨碎补总黄酮(assemble flavone of rhizome drynaria,AFDR)在修复大鼠颅骨缺损过程中对血清碱性磷酸酶、钙、磷及肌酐、谷丙转氨酶水平的影响。方法:60只6周龄雄性SD大鼠,饲养1周后Excel表随机分为两组(对照组和AFDR组),每组30只。用电动磨钻在大鼠左右颅骨钻孔建立颅骨缺损模型,右侧缺损可注射骨修复材料(injectable bone regeneration vomposite,IBRC)。术后第1天AFDR组中药灌胃,对照组去离子水灌胃。分别于术后2、4、8周采血,检测血清中碱性磷酸酶、磷、钙、肌酐、谷丙转氨酶指标,并进行统计学处理。结果:2周时碱性磷酸酶AFDR组高于对照组,4周时血清钙、磷及钙磷乘积AFDR组高于对照组。血清谷丙转氨酶各时间点两组差异无统计学意义。8周时血清肌酐AFDR组低于对照组。结论:骨碎补总黄酮在大鼠颅骨缺损修复过程中,于2～4周可影响碱性磷酸酶及血清钙磷水平,对肝肾无毒性。

Influence of assemble flavone of rhizome drynaria on the value of the blood serum alkalinity phosphatase,calcium,phosphorus in rats model with skull defects repair

Objective: To evaluate influence of assemble flavone of rhizome drynaria(AFDR) on the value of the blood serum alkalinity phosphatase(ALP),calcium(Ca),phosphorus(P),creatinine(Cr) and glutamic-pyruvic transaminase (GPT) in rats model with skull defects. Methods: Sixty SD male rats with age of 6-month were feeded for a week and then were randomly divided into control group and AFDR group,with 30 rats in each group. Left and right skull of rats were perforated with electromotive drill and the model of skull defects was made. Injectable bone regeneration vomposite(IBRC) was implanted right skull defects. The rats of control group and AFDR group were respectively lavaged with AFDR and deionized water at the first day after operation. The rats were respectively killed at the 2nd,4th and 8th week and the blood serum ALP,Ca,P,Cr,GPT were detected and analyzed by statistics. Results: At the 2nd week after operation,blood serum ALP in AFDR group was higher than that of control group. At the 4th week after operation,blood serum Ca,P,and calcium-phosphorus product in AFDR group was higher than that of control group;there was no significant difference in GPT between two groups. At the 8th week after operation,blood serum Cr in AFDR group was lower than that of control group. Conclusion: When AFDR is used in the repairing of bone defect for 2-4 weeks,it may affect the level of ALP,Ca,P,and without toxicity to liver and kindey.