Ozone production by amino acids contributes to killing of bacteria |
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Authors: | Yamashita Kouhei Miyoshi Takashi Arai Toshiyuki Endo Nobuyuki Itoh Hiroshi Makino Keisuke Mizugishi Kiyomi Uchiyama Takashi Sasada Masataka |
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Affiliation: | Departments of Hematology and Oncology and Anesthesia, Kyoto University Hospital, Kyoto 606-8507, Japan. |
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Abstract: | Reactive oxygen species produced by phagocytosing neutrophils are essential for innate host defense against invading microbes. Previous observations revealed that antibody-catalyzed ozone formation by human neutrophils contributed to the killing of bacteria. In this study, we discovered that 4 amino acids themselves were able to catalyze the production of an oxidant with the chemical signature of ozone from singlet oxygen in the water-oxidation pathway, at comparable level to antibodies. The resultant oxidant with the chemical signature of ozone exhibited significant bactericidal activity in our distinct cell-free system and in human neutrophils. The results also suggest that an oxidant with the chemical signature of ozone produced by neutrophils might potentiate a host defense system, when the host is challenged by high doses of infectious agents. Our findings provide biological insights into the killing of bacteria by neutrophils. |
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Keywords: | host defense singlet oxygen neutrophil chronic granulomatous disease |
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