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Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: A comparative study with green tea (Camellia sinensis)
Authors:Rossana M. Costa,Ana S. Magalhã  es,José   A. Pereira,Paula B. Andrade,Patrí  cia Valentã  o,Má  rcia Carvalho,Branca M. Silva
Affiliation:1. CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, R. Carlos da Maia, 296, 4200-150 Porto, Portugal;2. CIMO, Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-855 Bragança, Portugal;3. REQUIMTE, Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, Portugal;4. REQUIMTE, Serviço de Toxicologia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, Portugal
Abstract:This study aimed to determine the phenolic profile and to investigate the antioxidant potential of quince (Cydonia oblonga) leaf, comparing it with green tea (Camellia sinensis). For these purposes, methanolic extracts were prepared and phenolics content of quince leaf was determined by HPLC/UV. The antioxidant properties were assessed by Folin–Ciocalteu reducing capacity assay and by the ability to quench the stable free radical 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes.
Keywords:Antioxidant activity   Cydonia oblonga   Quince leaf   Hemolysis   Phenolic compounds   Camellia sinensis
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