基因芯片技术检测宫颈癌组织的人乳头瘤病毒基因型

目的:了解宫颈癌患者人乳头瘤病毒的感染率及基因型分布情况,探讨人乳头瘤病毒基因型与癌肿类型的相关性。方法:采用可检测23种HPV基因型的基因芯片方法检测200例宫颈癌组织的HPV基因型,计算HPV及各基因型的感染率,比较HPV及其主要基因型分布与癌肿类型的关系。结果:200例宫颈癌组织中HPV的阳性率为94.00%,其中宫颈鳞癌为95.86%,显著高于宫颈腺癌的80.00%,χ2=9.73,P<0.01;而HPV16、HPV18型和HPV双重感染在鳞、腺癌中的阳性率无显著性差异;200例宫颈癌组织中共检测到9种HPV基因型,分别为HPV16、18、45、33、58、59、73、31、56,HPV16检出率最高,达74.00%、其次是HPV18(16.00%);HPV双重感染24例,检出率达12.00%。结论:HPV在宫颈鳞癌的感染率显著高于宫颈腺癌;HPV基因型分布与癌肿类型无关;宫颈癌的HPV基因型别多样,且均为高危型感染,以16、18型最常见,并存在双重感染。

The study of human papillomavirus genotyping in cervical cancer

Objective:To investigate human papilloma virus (HPV) prevalence and HPV genotypes distribution in cervical cancer, and discuss the correlation of HPV genotypes and cancer types.Methods:HPV genotype analysis was performed through gene chip technique which could detect 23 genotypes of HPV in 200 cervical cancer, the infection rate of HPV and HPV genotype was calculated,and the relation of HPV genotypes and cancer types was discussed..Results:①A total of 94% cervical cancer cases were HPV infected positive. The HPV infection rate of squamous cancer and adencarcinoma was present 95.86% and 80.00% respectively, and there was a significant difference in these two types. ② 9 HPV genotypes were detected in 200 cases of cervical cancer, including HPV16, 18, 45, 33, 5, 59, 73, 31, 56. HPV16 (74%) was the most common type, the second was HPV18 (16.00%). In addition, 12.00% cervical cases were detected with double infection of HPV.Conclusion:HPV infection rate in squamous cancer is higher than that in adencarcinoma, but HPV genotypes distribution has no correlation with the cancer types. HPV genotypes are diversiform in cervical cancer tissue, all of which are high risk HPV, HPV16 and HPV18 are the most common type, HPV double infection could be found frequently.