脂质体携载精氨酸对大鼠钙化血管精氨酸/NO途径的影响

目的:观察钙化血管精氨酸/NO途径的改变和脂质体携载精氨酸对血管钙化及精氨酸/NO途径的影响。方法:维生素D3注射制备大鼠血管钙化模型,检测血管钙含量、血浆精氨酸和亚硝酸盐含量、血管环磷酸鸟苷(cGMP)含量、血管一氧化氮合酶(NOS)活性及血管精氨酸水平。结果:钙化组大鼠血管钙含量较对照组升高7.5倍(P<0.01),血浆NO生成减少,血管cGMP水平降低,血管NOS活性增高(P<0.01),但精氨酸含量明显减少(-19.1%,P<0.01)。脂质体携载精氨酸治疗后血管钙含量较钙化组降低51.2%(P<0.01),血浆NO生成增加,血管cGMP含量增加5.1%(P均<0.05),血管精氨酸含量增加15.7%(P<0.05)。结论:给予脂质体携载精氨酸治疗可以减轻大鼠的血管钙化程度,改善钙化血管L-Arg/NOS/NO/cGMP途径紊乱,提示脂质体携载精氨酸对防治动脉粥样硬化等疾病的血管钙化可能具有潜在临床意义。

Effects of Liposome-encapsulated Arginine on Calcified Vascular Arginine/NO Pathway in Rats

Objective: To observe the alternations of calcified vascular arginine/NO pathway and the effects of liposome-encapsulated arginine on vascular calcification and arginine/NO pathway.Methods: The vascular calcification model was produced by vitamin D_3.Vascular calcium contents,plasma arginine and nitrite contents,vascular cGMP contents,vascular nitric oxide synthase(NOS) activity and vascular arginine levels were detected.Results: Compared with the control group,the vascular calcium contents of the rats in vascular calcification group increased 7.5 times((P<)0.01),plasma NO production reduced,vascular cGMP levels decreased,vascular NOS activity increased((P<)0.01).The arginine contents,however,obviously decreased(-19.1%,(P<)0.01).Compared with the calcification group,the vascular calcium contents of the rats injected by liposome-encapsulated arginine decreased 51.2%((P<)0.01),the productions of plasma NO increased,vascular cGMP contents increased 5.1%((P<)0.05),and the vascular arginine contents increased 15.7%((P<)0.05).Conclusions: Liposome-encapsulated arginine may attenuate the vascular calcification of the rats and improve the disturbance of calcified vascular L-arginine/NOS/NO/cGMP pathway.Thus,it appears some potential clinical value that liposome-encapsulated arginine to the vascular calcification of atherosclerosis and other cardiovascular diseases.