用RP-HPLC法测定人血浆中丙泊酚浓度

目的：建立测定人血浆中丙泊酚浓度的RP-HPLC法.方法：以麝香草酚（thymol）为内标,血浆中加入缓冲液（0.1 mol/L NaH2PO4）混匀后经环己烷萃取,吸取有机相,加入四甲基氢氧化铵-异丙醇稀释液,氮气吹干,以流动相甲醇-水（80∶20）溶解.色谱柱：Diamonsil C18柱（200 mm×4.6 mm,5μm）;流速：1 ml/min;检测波长：270 nm;柱温：30℃.结果：丙泊酚血药浓度在0.20～～ 10.00 μg/ml范围内线性关系良好（r=0.9997）,最低定量限为0.20 μg/ml.低、中、高3种浓度的方法回收率为98.37％～100.26％,提取回收率为83.47％～92.11％.日内RSD为4.72％～9.70％,日间RSD为4.27％～6.41％.结论：该方法适用于丙泊酚的人体药动学研究和血药浓度检测.

Determination of propofol concentration in human plasma by RP-HPLC method

Objective：To establish a RP-HPLC method for the determination of propofol concentration in human plasma.Methods：Thymol was used as internal standard,plasma was extracted with cyclohexane after it was added into the buffer solution （0.1 mol/L NaH2 PO4）.The organic phase was added into tetramethyl ammonium hydroxide and 2-propanol diluted solution,then was dried under the stream of nitrogen.The residue was dissolved by mobile phase of methanol-water （80：20）.Separation was performed on a Diamonsil C18 column （200 mm × 4.6 mm,5 μm） and the mobile phase was methanol-water（80 ∶ 20） with the flow rate of 1 ml/min.The column temperature was 30 C and detection wavelength was 270 nm.Results：Propofol presented a good linearity within the range of 0.20-10.00 μg/ml （r=0.999 7）.The lowest limit of quantitation was 0.20 pg/ml.The recovery rates of low,medium and high concentrations were 98.37 ％-100.26 ％ and extractive recovery rates were 83.47％-92.11％.It had good intra-day and inter-day precision.The RSD of intra-day was 4.72％-9.70％ and inter-day was 4.27％-6.41％.Conclusion：This method can be used in pharmacokinetic study and plasma level determination of propofol.