| YAP对胶质瘤细胞系U87MG细胞迁移的促进作用 |
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| 引用本文： | 包义君,王维,王鹏飞,仇波,吴安华,王运杰. YAP对胶质瘤细胞系U87MG细胞迁移的促进作用[J]. 神经疾病与精神卫生, 2013, 0(5): 453-456 |
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| 作者姓名： | 包义君 王维 王鹏飞 仇波 吴安华 王运杰 |
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| 作者单位： | 中国医科大学附属第一医院神经外科中国医科大学神经外科研究所,110001 |
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| 摘 要： | 目的评价Hippo信号传导通路下游转录辅助因子YAP对胶质瘤细胞系U87MG细胞迁移的作用。方法利用Westernblot法验证YAP在U87MG中的内源性表达，通过过表达YAP以及siRNA干扰技术分别外源性表达YAP及干扰内源性YAP表达，进而利用细胞划痕实验评价YAP在u87MG细胞迁移中的作用。结果Westernblot结果证实YAP在u87MG细胞中内源性表达；细胞划痕实验结果显示YAP过表达能够促进细胞的迁移，而干扰表达则阻碍细胞的迁移，与对照组比较差异均有统计学意义（P〈0．01）。结论YAP在胶质瘤细胞中表达而且在细胞迁移中发挥促进作用。
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| 关 键 词： | 胶质瘤 Hippo信号传导通路 YAP 细胞迁移 |
Promoting effect of YAP on migration of U87MG cells |
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| Affiliation: | BAO Yi-jun, WANG Wei, WANG Peng-fei, et al. Department of Neurosurgery, the First Hospital of China Medical University, Shenyang 110001, China |
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| Abstract: | Objective To investigate the role of Hippo signaling pathway downstream transcrip- tional co--activator YAP in the migration of U87MG cell line. Methods The endogenous expression of YAP protein was evaluated by Western blot. The wound healing assay was used to testify the migration ability of U87MG cell with YAP over--expression and interference expression separately. Results YAP over--expression enhanced the migration in the wound healing assay （P 〈 0.05）, otherwise, the inter- ference of YAP by siRNA inhibited the migration of U87MG （P 〈 0.05）. Conclusions YAP is ex- pressed endogenously in U87MG cell and plays a critical role in the migration of U87MG cells. |
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| Keywords: | Hippo pathway YAP Cell migration |
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