过敏性紫癜患儿血清基质金属蛋白酶-9的检测及意义

目的探讨基质金属蛋白酶-9（MMP-9）在过敏性紫癜（HSP）中的作用。方法采用双抗体夹心酶联免疫吸附（ELISA）方法测定40例HSP患儿不同时期的血清MMP-9水平。结果HSP急性发作组血清MMP-9及C-反应蛋白（CRP）水平升高，与正常对照组和缓解组比较，差异均有统计学意义（t分别=12-30、8．42、6．37、8．13，P均〈0．05）；HSP缓解组血清MMP-9仍高，与正常对照组比较，差异有统计学意：L（t=9．92，P〈0．051：HSP急性期并发肾损害组及有胃肠道症状组患儿血清MMP-9水平升高。与无肾损害组及无胃肠道症状组比较，差异有统计学意义（t分别：3．75、3．17，P均〈0．05），而血清CRP水平差异无统计学意义（t分别=0．96、0．56，P均〉0．05）。HSP急性期患儿血MMP-9水平与CRP浓度之间成直线正相关（r=0．68，P〈0．05）。结论MMP-9参与了HSP疾病的病理生理过程。血清MMP-9测定对HSP．尤其是紫癜性肾炎及胃肠道并发症的临床诊断、病情评估及指导治疗有一定帮助。

Determination and clinical significance of matrix metalloproteinase-9 in children with henoch-schonlein purpura

Objective To investigate the role of serum matrix metalloproteinase-9 in children with henochschonlein purpura. Methods The serum concentration of MMP-9 was measured by enzyme-linked immunosorbent assay method in 40 acute SHP patients, 35 recovery patients and 30 healthy controls. Results The serum levels of MMP-9 and C-reactive protein in acute phase were significantly higher than those in healthy controls and those during recovery period（t=12.30,8.42,6.37,8.13 ,P 〈 0.05）. The serum level of MMP-9 in the HSP patients dropped back slowly and their levels during recovery period was still higher than those in healthy controls （t=9.92,P〈0.05）. The serum levels of MMP-9 were higher in renal damage and gastrointestinal symptoms groups than those in non-renal damage and non-gastrointestinal symptoms groups （t=3.75,3.17,P〈0.05）, whereas the serum level of CRP had no significant difference between them （t=0.96,0.56,P〉0.05）. The serum level of MMP-9 showed positive correlation with the level of CRP （r =0.68, P 〈0.05）. Conclusions The damage of extracellularmatrix plays an important role in the pathological mechanism of HSP. The level of MMP-9 may be used as index of the HSP activity and progression as well as the damage of gastrointestinal and renal system in HSP.