酒精对骨髓基质细胞增殖及分化的影响

目的 观察酒精对骨髓基质细胞增殖及分化的作用．探讨骨质疏松的病理学机理。方法 以0.09mol/L酒精加入骨髓基质细胞培养物中，测定增殖的骨髓基质细胞数及培养液中骨钙素含量。通过苏丹Ⅲ脂肪细胞染色计数观察酒精作用时间对脂肪细胞分化的影响。结果 实验组骨髓基质细胞数及培养液中骨钙素含量明显低于对照组，脂肪细胞的数量随酒精作用时间延长而增多。结论 酒精抑制骨髓基质细胞增殖及向成骨方向分化，促进其向脂肪细胞分化，这可能与酒精中毒引起继发性骨质疏松时骨量减少、髓内脂肪组织增多有关。

Effect of alcohol on marrow stromal cell proliferation and differentiation

Objective To study the effect of alcohol on proliferation and differentiation of marrow stromal cells, and on pathogenetic mechanism of osteoporosis. Methods Proliferation of marrow stromal cells treated with alcohol and contents of osteocalcin in the media were assessed. Results The number of the marrow stromal cells and the contents of osteocalcin in the media in the experimental groups were significantly lower than those in the control group. The adipocytes increased in number with increasing duration of exposure to alcohol. Conclusion Alcohol inhibits marrow stromal cell proliferation and osteoblastic differentiation and promotes its differentiation into adipocytes. This may be a major contributing factor of bone loss and increase in marrow fat volume in osteoporosis.