反相高效液相色谱法测定如意金黄贴膏中小檗碱的含量

 目的：黄柏在如意金黄贴膏中为君药，建立黄柏中小檗碱的定量方法。方法：采用反相高效液相色谱法测定小檗碱的含量。使用μ-BondapakTM C₁₈(3.9 mm×300 mm，10 μm)色谱柱，以乙腈-磷酸二氢钾-磷酸（300∶700∶1.50）为流动相，检测波长为350 nm。结果：此法线性关系良好，平均回收率为100.56%。结论：本方法精密度高，分离度良好，可用于如意金黄贴膏的质量控制。

Determination of berberine in Ruyi Jinhuang Tiegao by RP-HPLC

OBJECTIVE Cortex Phellodendri(Huangbo) is one of the monarch drugs in Ruyi Jinhuang Tiegao.To gurantee the quality of this drug,a RP-HPLC method was established to determine the active constituent-Berberine in Cortex Phellodendri.METHODS The experiment conditions were to use μ-BondapakTM C18(3.9 mm×300 mm,10 um) column,using CH₃CN:KH₂PO:H₃PO₄ (300∶700∶1.50)as a mobile phase and detection wavelength at 350 nm.RESULTS The linearity of this method was well and the average recoveries were 100.56%.CONCLUSIONS This mothod shows high accuracy,good seperating degree and can be used for quality control of Ruyi Jinhuang Tiegao.