蛋白激酶C信息通道与阻塞性黄疸患者免疫功能评估的关系

目的研究蛋白激酶C(PKC)信息通道在阻塞性黄疸(阻黄)患者机体发病机理中的作用. 方法从51例阻黄患者和16例正常对照者的外周静脉血中分离和纯化淋巴细胞胞浆及胞膜部分PKC,然后采用同位素(γ-32P-ATP催化活性测定法检测它们的活性.结果(1)阻黄患者外周血淋巴细胞PKC的总活性较正常健康人明显增强(r=0.64,P＜0.01),且胞膜部分PKC活性占总活性的百分值较正常组也明显升高(r=0.63,P＜0.05) .(2)阻黄患者淋巴细胞PKC的活性与血清黄疸的程度(T-BIL)显著正相关(r=0.67,P＜0.01),与血清可溶性白介素-2受体(sIL-2R)浓度显著正相关(r=0.58,P＜0.01).结论 PK C信息通道的激活与黄疸程度有关,且可能参与了阻黄T淋巴细胞的活化,在阻黄机体的免疫调节及病情评估中可能有重要意义.

The relationship between protein kinase C signal pathway and the immunologic evaluation in patients with obstructive jaundice

OBJECTS: To explore the roles of the protein kinase C (PKC) signal pathway in immunologic evaluation of patients with obstructive jaundice. METHODS: PKC from cytosolic and membrane fractions of peripheral blood lymphocytes (PBL) was isolated and purified, then its activities were determined by radioactive isotope gamma-32P-ATP-catalyzing assay. RESULTS: Total PKC activities in PBL in patients with obstructive jaundice were significantly (r = 0.64, P < 0.01) increased as compared to those in normal subjects. Moreover, the membrane PKC activities and their percentages of the total PKC activities were higher in the patients with obstructive jaundice than in normal subjects (r = 0.63, P < 0.05). The total PKC activities in PBL were significantly correlated with the levels of soluble IL-2 receptor (sIL-2R) (r = 0.67, P < 0.01) and the degree of jaundice (T-BIL) (r = 0.58, P < 0.01) in serum. CONCLUSIONS: The activities of the PKC signal pathway is related to the degree of T-BIL. PKC may be implicated in the activation of T-lymphocytes in patients with obstructive jaundice. It is suggested that the role of the PKC signal pathway may be important in the immune regulation and the assessment of pathosis in patients with obstructive jaundice.