预制检测板MTT法检测癌患者外周血淋巴细胞化疗药物敏感性的研究

目的:基于众多文献报道MTT法化疗药敏测定中癌细胞抑制率与外周血淋巴细胞抑制率呈正相关,探讨预制检测板MTT法在检测癌患者外周血淋巴细胞药物敏感性中的价值,以推动检测标准化与指导临床化疗用药。方法:应用泰伦公司生产的MTT法肿瘤细胞药敏预制检测板,对75例癌患者外周血淋巴细胞进行20种药物的药敏检测,操作方法按预制检测板使用说明书。结果:39例肺癌患者外周血淋巴细胞对化疗药物敏感性,由高到低前5名依次为:卡氮芥(BCNU),紫杉醇(PTX),阿霉素(ADM),长春瑞滨(NVB)和表阿霉素(eADM);25例乳腺癌患者外周血淋巴细胞对化疗药物敏感性,前5名依次为:BCNU,PTX,NVB,ADM和米托蒽醌(MIT);11例胃癌患者外周血淋巴细胞对化疗药物敏感性,前5名依次为:PTX,ADM,BCNU,eADM和阿糖胞苷(Ara-C)。结论:该MTT法预制检测板适用于癌患者药物敏感性试验,对临床癌患者个体化化疗有重要指导意义。

Study on MTT Prefabricated Plate for Chemosensitivity in Peripheral Blood Lymphocyte with Cancer Patients

Objective Based on lots of reports of MTT chemosensitivity assay demonstrating that the inhibited rates between cancer cells and peripheral blood lymphocytes showed positive correlation,to explore the value of the MTT prefabricated plate in peripheral blood lymphocytes for three kinds of cancer patients.Methods Using Talent MTT prefabricated plate,75 peripheral blood lymphocyte samples from three kinds of cancer patients were analyzed by chemosensitivity assay with 20 anticancer drugs.Results To 39 lung cancer patients,5 anticancer drugs had higher sensitivity and were BCNU,PTX,ADM,NVB and eADM;to 25 breast cancer patients,the 5 anticancer drugs were BCNU,PTX,NVB,ADM and MIT;to 11 gastric cancer patients,they were PTX,ADM,BCNU,eADM and Ara-C.Conelusion The MTT prefabricated plate is applicable for the chemosensitivity assessment of peripheral blood lymphocytes with the cancer patients and is of important reference value to choose chemotherapeutic drugs in clinic.