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| 人原代胶质瘤细胞自杀基因治疗体外研究 | |
| 引用本文： | 陶慧敏,李承晏,余绍祖. 人原代胶质瘤细胞自杀基因治疗体外研究[J]. 卒中与神经疾病, 1999, 6(1): 9-10 |
| 作者姓名： | 陶慧敏 李承晏 余绍祖 |
| 作者单位： | [1]湖北医科大学附属同仁医院神经科 [2]湖北医科大学附属第一医院神经科 |
| 摘 要： | 目的：应用原代培养的胶质瘤细胞作不研究对象，观察ＡＣＶ对转染了ＨＳＶ－ｔｋ基因的肿瘤细胞的杀伤作用。方法：表达单纯疱疹病毒胸苷激酶（ＨＳＶ－ｔｋ）重组逆转录病毒载体ＳＴＫ，经包装细胞ＰＡ３１７包装成重组逆转录病毒，ＮＩＨ３Ｔ３细胞测定病毒滴度，用此病毒感染原代培养的人胶质瘤细胞，以Ｇ４１８选择后获得ＴＫ＋肿瘤细胞。ＭＴＴ法及活细胞动态观察ＡＣＶ对转基因细胞的毒性作用。结果：ＡＣＶ对ｔｋ＋胶质瘤细胞 |
| 关 键 词： | 单纯疱疹病毒 胸苷激酶 基因疗法 胶质瘤 |
Study HSV tk gene mediated killing of human glioma |
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| Tao Huimin,Li Chengyan,Yu Shaozu,et al.. Study HSV tk gene mediated killing of human glioma[J]. Stroke and Nervous Diseases, 1999, 6(1): 9-10 | |
| Authors: | Tao Huimin Li Chengyan Yu Shaozu et al. |
| Affiliation: | Tao Huimin,Li Chengyan,Yu Shaozu,et al. The First Affiliated Hospital of Hubei Medical University Wuhan 430060 |
| Abstract: | Objective:The human primary cultrue glioma.Methods:The HSV tk gene was transfered into primary culture glioma cell by retroviral vector.The cellular morphology and the killing effect of ACV were observed in both tk glioma cell and tk- glioma cell.Results:The results showed that tk glioma cell was significantly killed by ACV compared with nontransfected cell ( P <0.002) and the killing effect increased as the ACV concentration increased ( P <0.001).Conclusion:The human primary culture glioma transfected with HSV tk gene by retroviral vector can be killed by ACV selectively. |
| Keywords: | HSV tk gene therapy glioma primary culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |