应用高效液相色谱法检测男性不育患者精浆丙二醛水平

目的:探讨高效液相色谱法(HPLC)检测精浆中脂质过氧化产物丙二醛(MDA)水平的意义。方法:93例不育男性分为:阻塞性无精子症组(12例);非阻塞性无精子症组(15例);少精子症组(21例);弱精子症组(19例);少弱精子症组(16例);少弱畸精子症组(10例)。18例生育男性作为对照组。采用HPLC检测生育与不育男性精浆MDA含量。结果:生育组精浆MDA值除与阻塞性无精子症组差异无显著性(P>0.05)外,与其他各组相比均有极显著性差异(P均<0.01)。各不育男性组间精浆MDA值也有统计学差异。结论:应用HPLC检测精浆MDA水平是诊断活性氧产生过高所致男性不育的一个重要指标。

Detection of Malondialdehyde Concentrations in Seminal Plasma of Infertile Men by High-performance Liquid Chromatography

Objective: To detect concentrations of malondialdehyde (MDA), product of lipid peroxidation in seminal plasma, and evaluate its significance. Methods: Ninety-three cases of infertile patients were divided into obstructive azoospermic group (12 cases), non-obstructive azoospermic group ( 15 cases), oligozoospermic group (21 cases), asthenozoospermic group (19 cases), oligoasthenozoospermic group (16 cases), and oligoasthenoteratozoospermic group (10 cases). Eighteen fertile males were control group. MDA concentrations of seminal plasma in fertile and infertile men were assessed by high-performance liquid chromatography (HPLC). Results: Exception of obstructive azoospermic group, MDA concentrations of seminal plasma in control group had very significant difference with those in other infertile groups (P<0.01). MDA concentrations of seminal plasma had significant difference between infertile guoups. Conclusion: Detection of MDA concentrations in seminal plasma by HPLC has important significance in diagnosis of male infertility induced by overproduction of reactive oxygen species in male reproductive system.