间歇低氧对大鼠海马神经细胞自噬相关蛋白表达的影响

目的通过模拟阻塞性睡眠呼吸暂停低通气综合征的发病特征,建立大鼠间歇性低氧(IH)模型,观察IH后大鼠海马CA1区神经细胞线粒体自噬及相关蛋白的表达。方法 72只雄性Wistar大鼠随机分为对照组36只和间歇低氧(5%IH)组36只,对照组向低氧箱内持续注入压缩空气,5%IH组每天放入低氧箱内IH暴露7h,模型完成后分别于1、3、5、7、10和14d采用透射电镜观察大鼠海马CA1区神经细胞线粒体超微结构的改变;免疫组织化学法检测Beclin-1和微管相关蛋白1轻链3(LC3)的表达。结果与对照组比较,5%IH组3d开始出现线粒体超微结构的明显改变,可见线粒体自噬体形成;1、3、5、10和14dBeclin-1及LC3蛋白表达均明显升高(P<0.05),于10d达高峰(P<0.05),14d开始下降,差异有统计学意义(P<0.05)。结论 IH早期可诱导大鼠海马神经细胞线粒体发生自噬及自噬相关蛋白Beclin-1和LC3的表达。

Effect of intermittent hypoxia on expression of nerve cell autophagy-related protein in hippocampus of rats

Objective To study the effect of intermittent hypoxia(IH)on expression of nerve cell autophagy-related protein in hippocampus of rats by simulating the features of OSAHS.Methods Seventy-two male Wistar rats were randomly divided into control group(n=36)and 5%IH group(n=36).Rats in control group received compressed air and those in 5%IH group were exposed to IH for 7heach day.On days 1,3,5,7,10,14 after the model was established,ultrastructure of rat hippocampal CA1 pyramidal cell mitochondria was observed under transmission electron microscope.Expression of Beclin-1and LC3 was detected by immunohistochemistry assay.Results The ultrastructure change of rat hippocampal CA1 pyramidal cell mitochondria was more significant in5%IH group than in control group,and mitochondria autophagosome formation was observed on day 3.The expression level of Beclin-1and LC3 protein was significantly higher in 5%IH group than in control group(P<0.05),reached its peak on day 10(P<0.05)and began to decrease on day 14(P<0.05).Conclusion Early IH can induce nerve cell autophagy and expression of autophagy-related Beclin-1and LC3 protein in hippocampus of rats.