荷瘤小鼠扶正抑瘤颗粒含药血清对H22细胞的凋亡、自由基含量和线粒体膜电位的影响

目的观察荷瘤小鼠扶正抑瘤颗粒（FYG）含药血清对H22细胞的凋亡、自由基含量和线粒体膜电位的影响。方法应用流式细胞仪检测FYG含药血清对H22细胞凋亡情况的影响；应用激光共聚焦显微镜检测H22细胞DNARNA、自由基含量和线粒体膜电位的变化。结果FYG含药血清可引起H22细胞的凋亡，细胞DNARNA和线粒体膜电位降低，自由基含量升高。结论FYK含药血清引起H22细胞的凋亡可能与其影响H22细胞自由基含量和线粒体膜电位变化有关。

Effect of Fuzheng Yiliu Granule Contained-serum from Tumor Bearing Mice on Apoptotic Rate, Free Radicals Content and Mitochondrial Membrane Potential of Hepatoma Cell Lines H22 in vitro

OBJECTIVE: To observe the effect of traditional Chinese herbs Fuzheng Yiliu Granule (FYG)-contained serum from tumor bearing mice on apoptotic rate, free radicals content and mitochondrial membrane potential of hepatoma cell lines H22 in vitro. METHODS: The effect of FYG drug-serum on apoptosis of hepatoma cell line H22 was determined using flow cytometry. The changes of DNA RNA, free radicals and mitochondrial membrane potential (delta psi m) in H22 cell were detected through laser scanning confocal microscope. RESULTS: FYG-contained serum can induce the apoptosis of H22 cell, enhance the free radicals content, and reduce the content of DNA RNA and delta psi m of H22 cell in vitro. CONCLUSION: The apoptosis of hepatoma cell line H22 induced by FYG is probably correlated to the change of free radicals content and delta psi m.