骨髓来源成骨细胞复合纳米晶羟基磷灰石胶原构建组织工程骨的实验研究

目的 :探讨纳米材料作为组织工程骨基质材料的可行性。方法 :分离培养兔骨髓间充质干细胞 ,诱导为成骨细胞后作为种子细胞 ,与纳米晶羟基磷灰石胶原材料于体外联合培养 ,复合物植入兔桡骨节段性缺损处 ,通过大体观察、HE染色及X线摄片了解成骨情况。结果 :兔骨髓间充质干细胞在体外可以大量扩增 ,能定向诱导为成骨细胞 ;复合物植入 16周后 ,X线摄片中可见桡骨缺损处连接良好。结论 :纳米晶羟基磷灰石胶原材料是组织工程骨的较好的支架材料。

Experimental Study of Tissue Engineering's Bone Establish of Osteoblasts From Bone Marrow Co-culture with the Nanometer Crystal Hydroxyapatite Collagen Material

Objective:To investigate the feasibility of the nanometer crystal hydroxyapatite collagen material as scaffold material in bone tissue engineering. Methods:Osteoblasts, which were induced from mesenchymal stem cells isolated from rabbits, bone marrow, were cultured as the seeding cells, then the cells were cocultured with the nanometer crystal hydroxyapatite collagen material in vitro. The cell-material complex was inserted in segment defect of rabbit's radius.Observation with lightmicroscope, HE staining and X ray photographs. Results:It is possible to induce mesenchymal stem cells, isolated from bone marrow, into osteoblasts. Sixteen weeks after cell-material complexer inserted, segment defect of rabbit's radius was connect well under X ray photographs. Conclusions:The nanometer crystal hydroxyapatite collagen material is a good scaffold material for the remodeling bone in bone tissue engineering.