不同类型的培养液对第一代试管婴儿成功率的影响

目的：体外受精、胚胎移植（IVFET）通常被称为第一代试管婴儿，本文探讨不同类型的培养液对体外受精、胚胎移植的影响，也就是对第一代试管婴儿（IVF）成功率的影响，以便改善更适宜的培养液体系。方法：对60例（61个周期）不孕症患者的临床资料进行分析。将61个行IVF周期患者分为三组，I组20个周期使用单纯EBSS（Eare’s平衡盐溶液）培养液为①；U组21个周期使用HTF、IVCONE序列培养液，且添加血清替代品SSS为②；III组20个周期使用HepesHTF、1VCONE序列培养液，也添加血清替代品SSS为③。体外操作环境相同：保湿、维持PH恒定、37℃恒温、渗透压和光线等设施为④。比较3组的受精率、卵裂率和临床妊娠率。结果：I组、Ⅱ组与Ⅲ组的受精率和卵裂率分别为：73．99％、75．74％、78．77％；71．52％、75．84％、78．44％；3组无明显差别，而临床妊娠率分别为10．oo％、42．85％、60．00％有明显差别。结论：序列培养液改善了胚胎的培养液体系，明显提高了第一代试管婴儿的成功率。

Effect of different kinds of culture medium on the success rate of the first generation test-tube baby

Objectives： In vitro fertilization , embryo transfer（ IVF ET） is often called the first generation test -tube baby , this paper discusses the effect of different types of culture mediun on in - vitro fertilization and embryo transfer, or on the success rate of the first generation test - tube baby with an aim to improve the culture medium. Methods： The clinical data of 60 infertil- ity patients （61cycles） were analyzed and the patients with 61 IVF cycles were divided into three groups , group I ： 20 cycles ad- ministered with a simple EBSS （Eare＇ s balanced salt solution） culture liquid ;group 1 ： 21 cycles administered with HTF,IVC sequence of ONE culture liquid, and added with serum substitutes for the SSS; group III： 20 cycle administered with Hepes HTF, IVC ONE series medium, and added with serum substitutes for the SSS. In vitro environment was kept similar for the three groups ： constant PH and moisture level, constant temperature of 37 ℃, similar osmotic pressure and light as well as other facili- ties. The three groups were compared in their fertilization rate, cleavage rate and clinical pregnancy rate. Results： The fertiliza- tion and cleavage rates for group I , II and m were respectively 73.99% ,75.74% ,78.77% ;71.52% ,78.44% ,75.84% ; The three groups had no significant difference in the two rates. However, clinical pregnancy rates werel0% ,42.85% ,60% respec- tively , three groups showing significant differences. Conclusion： The sequence of culture solution improves embryo culture me- dium system, and significantly enhances the IVF success rates of the first generation.