乙酰肝素酶与绒毛膜癌侵袭能力的关系

目的 通过研究乙酰肝素酶（Hpa）在绒毛膜癌高、低侵袭力细胞系及正常早孕绒毛组织中的表达情况,探讨Hpa与绒毛膜癌侵袭能力的关系。方法 通过Matrigel体外侵袭实验检测不同侵袭力绒毛膜癌细胞系JEG-3和JAR的体外侵袭能力;应用免疫细胞化学方法及蛋白印迹法（westem blot）检测Hpa蛋白在不同绒毛膜癌细胞系及正常早孕绒毛组织中的表达,并进行相关性分析。结果 （1）JEG-3细胞的穿膜细胞数[（191±17）个]较JAR细胞的穿膜细胞数[（106±13）个]明显增多,两者比较,差异有统计学意义（P〈0．05）。（2）Hpa蛋白在绒毛膜癌细胞JEG-3、JAR中均有表达,且主要定位于细胞质。（3）在正常早孕绒毛组织、JEG-3细胞和JAR细胞中均可检测到Hpa蛋白,JEG-3细胞中Hpa蛋白的表达量（1．560±0．180）明显高于JAR细胞Hpa蛋白的表达量（0．610±0．170）,绒毛膜癌细胞系中Hpa蛋白的表达量又显著高于正常早孕绒毛组织Hpa蛋白的表达量（0．190±0．008）,两两比较,差异均有统计学意义（P〈0．05）。（4）相关性分析显示,Hpa蛋白的表达量与绒毛膜癌细胞体外侵袭能力呈正相关关系（r=0．89,P〈0．05）。结论 Hpa蛋白在绒毛膜癌细胞中表达较正常绒毛组织高;Hpa蛋白在滋养细胞中的表达随滋养细胞侵袭能力的增加其表达上调;滋养细胞产生过量的Hpa对基底膜蛋白的水解作用在绒毛膜癌的侵袭、转移中发挥重要作用。

Correlation between expression of heparanase and invasion of choriocarcinoma

OBJECTIVE: To investigate the association between the expression of heparanase (Hpa) and the invasion of choriocarcinoma by studying the expression of Hpa in human choriocarcinoma cell lines JEG-3 and JAR and human chorionic villous tissues. METHODS: (1) Matrigel invasion assays were used to detect in vitro invasive ability of JEG-3 cells and JAR cells. (2) Expression of Hpa protein in the human chorionic villous tissues and choriocarcinoma cell lines (JEG-3 cells and JAR cells) were detected by immunocytochemistry and western blot. RESULTS: (1) The invasive cell number was significantly larger in JEG-3 cells than in JAR cells (191 +/- 17 vs 106 +/- 13, P < 0.05). (2) Hpa protein mainly located in cytoplasm by immunocytochemistry. (3) Hpa protein expression was stronger in JEG-3 cells than in the JAR cells (1.560 +/- 0.180 vs 0.610 +/- 0.170, P < 0.05); the Hpa protein expression was significantly stronger in choriocarcinoma cell lines than in human chorionic villous tissues (0.190 +/- 0.008) by western blot (P < 0.05). (4) The data suggested that there were significantly positive correlations between Hpa and the invasiveness of choriocarcinoma cells (r = 0.89, P < 0.05). CONCLUSIONS: (1) Hpa protein expression is significantly stronger in choriocarcinoma cell lines than in the human chorionic villous tissues. (2) Activation of Hpa enhances the invasion capability of choriocarcinoma. (3) Overexpression of Hpa may be related to the oncogenesis of choriocarcinoma and Hpa may play an important role in invasion of choriocarcinoma.