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| 检测微卫星序列多态性位点鉴别完全性葡萄胎的亲代来源 | |
| 引用本文： | Deng SS,Ding ZM,Qian JH,Lü WG,Wan XY,Xie X. 检测微卫星序列多态性位点鉴别完全性葡萄胎的亲代来源[J]. 中华妇产科杂志, 2007, 42(7): 468-471 |
| 作者姓名： | Deng SS Ding ZM Qian JH Lü WG Wan XY Xie X |
| 作者单位： | 1. 浙江省绍兴市第二医院妇产科,312000 2. 浙江大学医学院附属妇产科医院妇瘤科,杭州,310006 |
| 基金项目： | 基金项目：浙江省医药卫生科学研究基金（2005A061） |
| 摘 要： | 目的通过检测9个微卫星序列多态性位点以鉴别完全性葡萄胎的亲代来源。方法用多重PCR技术和变性聚丙烯酰胺凝胶电泳结合银染显色方法,对50例病理诊断为完全性葡萄胎患者的葡萄胎标本及夫妇外周血标本进行DNA分析。结果50例完全性葡萄胎患者中,双亲来源完全性葡萄胎7例,占14％;孤雄完全性葡萄胎43例,占86％,其中孤雄单精完全性葡萄胎35例,孤雄双精完全性葡萄胎8例。结论微卫星序列多态性位点检测是一种鉴别完全性葡萄胎亲代来源的简便、快速、可靠、高效的方法。 |
| 关 键 词： | 葡萄胎 串联重复序列 多态性 限制性片段长度 |
| 修稿时间： | 2007-01-25 |
Study on parental origin of complete hydatidiform mole detected by polymorphisms of microsatellite |
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| Deng Shun-sheng,Ding Zhi-ming,Qian Jian-hua,Lü Wei-guo,Wan Xiao-yun,Xie Xing. Study on parental origin of complete hydatidiform mole detected by polymorphisms of microsatellite[J]. Chinese Journal of Obstetrics and Gynecology, 2007, 42(7): 468-471 | |
| Authors: | Deng Shun-sheng Ding Zhi-ming Qian Jian-hua Lü Wei-guo Wan Xiao-yun Xie Xing |
| Affiliation: | Department of Gynecological Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China. |
| Abstract: | OBJECTIVE: Through detection of 9 loci of polymorphisms of microsatellite to investigate the parental origin of complete hydatidiform mole. METHODS: Using the technology and method of multipolymerase chain reaction and electrophoresis in denatured polyacrylamide gel and silver stain detection, we carried out DNA analysis on 50 cases of complete hydatidiform mole diagnosed by histopathology and 50 copies of the couples' peripheral blood. RESULTS: There are 7 cases of biparental complete hydatidiform mole (14%); and 43 cases of androgenetic complete hydatidiform mole (86%) among 50 cases of complete hydatidiform mole. CONCLUSIONS: Detection of polymorphisms of microsatellite is a technology that can be used to identify the parental origin of complete hydatidiform mole. It is simple, quick, reliable and highly efficient. |
| Keywords: | Hydatidiform mole Tandem repeat sequences Polymorphism,restriction fragment Iength |
| 本文献已被 维普 万方数据 PubMed 等数据库收录！ | |