青蒿琥酯抑制人肝癌细胞株HepG2细胞生长及其机制的初步研究

目的：研究青蒿琥酯对人肝癌细胞株HepG2细胞增殖和survivin表达的影响,探讨青蒿琥酯影响肝癌细胞增殖的可能机制.方法：常规培养人肝癌细胞株HepG2,设立空白对照组;青蒿琥酯不同浓度组（3.125μg/ml、6.25μg/ml、12.5μg/ml、50μg/ml）,作用不同时间后用MTT法检测青蒿琥酯对人肝癌细胞株HepG2细胞增殖的影响,应用RT-PCR检测细胞survivin在mRNA水平的表达.结果：青蒿琥酯作用人肝癌细胞株HepG2后,与对照组比较细胞生长明显受到抑制（P〈0.05）,当青蒿琥酯浓度在12.5μg/ml时,survivin的表达（相对表达量为（0.75＋0.01）显著降低（P〈0.05）.结论：青蒿琥酯可通过下调survivin的表达来抑制肝癌细胞HepG2的生长.

Primary research on artesunate inhibiting human hepatoma cell lines HepG2 cells growth and its mechanisms

Objective：Its aimed to investigate the effects of artesunate on proliferation and surviving expressions of human hepatoma cell lines HepG2,human hepatoma cell lines HepG2 cultivated by convention, were randomly divided into 2 groups：untreated control group; different concentration artesunate treated with HepG2. And then, the proliferation of HepG2 were detected by MTT; After treated with different concentration for 48 hours,using RT-PCR method to detect survivin expression at the mRNA level. Results：Compared with the control group, the proliferation of HepG2 was significantly inhibited by artesunate（P 〈 0.05） ;when the concentration of artesunate reach to 12.5μg/ml, the survivin expressionin（relative expression levels of 0. 75±0. 01） were significantly lower（P 〈 0.05）. Conclusion： Artesunate can inhibited the proliferation of H epG2 through reducing the expressionin of the surviving.