应用羧化胶乳凝集试验诊断人、畜布鲁氏菌病的研究

应用以化学交联法制备的布鲁氏菌１６Ｍ抗原－羧化胶乳制剂的羧化胶乳凝集试验（ＬＡＴ）、试管凝集试验（ＳＡＴ）、虎红平板凝集试验（ＲＢＰＴ）以及酶联免疫吸附试验（ＥＬＩＳＡ）、半胱氨酸凝集试验（ＣＹＴ），对在四省布鲁氏菌病疫区收集的部分人、畜血清中布氏菌抗体进行对比检测。结果表明，ＬＡＴ较之ＲＢＰＴ具有更高的特异性；与ＳＡＴ相比，无论阳性或阴性符合率均较一致。另外，ＬＡＴ可检测血清中ＩｇＧ、ＩｇＭ两类布氏菌特异性抗体。因而本试验对人、畜布病的诊断具有特异、敏感和简便的优点，更宜在基层推广应用。

Study on Carboxylated Latex Agglutination Test for Serodiagnosis of Human and Animal Brucellosis

carboxylated latex agglutination test was developed for detecting antibodies in sera from 372 humanand animal brucelIosis.Of which 94 sera from human was detected by ELISA. The latex was chemical-linkedwith B. melitensis 16M and extracted with 5% NaCl.Comparing with RBPT in detecting antibodies with bru-cellosis,the LAT was more stable and specific;the positive or negative results detected by LAT were consis-tent with those by SAT and ELISA. EAT may detect both IgG and IgM antibodies to Brucella in Human and animal.It is concluded that LATis stable,specific,sensitive and practicible for the serodiagnosis of brucellosis.