Laboratory diagnosis of specific antibody deficiency to pneumococcal capsular polysaccharide antigens by multiplexed bead assay |
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Authors: | Heleen Borgers Leen Moens Capucine Picard Axel Jeurissen Marc Raes Kate Sauer Marijke Proesmans Kris De Boeck Jean-Laurent Casanova Isabelle Meyts Xavier Bossuyt |
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Affiliation: | 1. Experimental Laboratory Medicine, Department of Medical Diagnostic Sciences, Biomedical Sciences Group, Catholic University Leuven, Leuven, Belgium;2. Laboratory of Human Genetics of Infectious Diseases, Necker Branch, Institut National de Santé et de Recherche Médicale, U550, Paris, France;3. University Paris Descartes, Necker Medical School, Paris, France;4. Department of Pediatrics, University Hospital Leuven, Belgium;5. Lab of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, NY, USA |
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Abstract: | We evaluated a multiplexed bead-based assay (xMAP® Pneumococcal Immunity assay from Luminex) for the simultaneous determination of antibodies against 14 capsular polysaccharides. Post-vaccination (Pneumovax®) antibody concentrations were measured in 35 healthy children, 40 healthy adults, 99 consecutive patients with increased susceptibility to respiratory infection, and 24 patients with a deficient anti-polysaccharide antibody response. The serotype-specific lower 5th percentile (cutoff) value for the post-immunization antibody concentration was determined in healthy individuals. Eleven of 99 patients (11%) failed to mount a response that was > 5th percentile of controls for at least 6 of the 14 serotypes tested, whereas only 3 of 75 controls (4%) failed to do so. All patients with known anti-polysaccharide antibody deficiency failed to mount a response that was > 5th percentile of controls for at least 6 of the 14 serotypes tested. The XMAP® pneumococcal immunity panel appears useful for identifying individuals with a low response to the unconjugated pneumococcal vaccine. |
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Keywords: | Caps-PS capsular polysaccharides |
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