注水瓶连续使用时间对其中水细菌污染情况的影响

目的 探讨注水瓶的连续使用时间。方法 采用前瞻性研究方法监测重庆市某三甲医院内镜中心的5套注水瓶，2019年7月—2019年11月将注水瓶灌注无菌水，于第1、2、3、4、5天每日诊疗结束采集水标本进行微生物学检测，监测注水瓶水细菌种类及细菌菌落数合格率。结果 注水瓶水在第1、2、3、4、5天的细菌菌落数合格率分别为96.0%、82.0%、76.0%、70.0%、38.0%，差异有统计学意义（P<0.001）。共分离69株细菌，均为条件致病菌，以解甘露醇罗尔斯顿菌、铜绿假单胞菌较多，分别为29、10株。结论 医疗机构应充分重视内镜诊疗用水的微生物污染，注水瓶水已成为诊疗水的潜在污染源，为降低内镜诊疗安全隐患，注水瓶连续使用不得超过1 d。

Effect of continuous use time of water injection bottles on microbial contamination of water for endoscope

Objective To investigate the continuous use time of water injection bottles. Methods Five sets of water injection bottles in the endoscopy center of a tertiary first-class hospital in Chongqing was monitored through prospective study, sterile water was injected into the water injection bottles from July 2019 to November 2019, water was collected for microbiological test on the 1st, 2nd, 3rd, 4th and 5th days after diagnosis and treatment every day, bacterial species and qualified rate of bacterial colony count in water injection bottle was monitored. Results Qualified rates of bacterial colony count on the 1st, 2nd, 3rd, 4th, and 5th days were 96.0%, 82.0%, 76.0%, 70.0%, and 38.0% respectively, difference was statistically significant (P<0.001). A total of 69 strains of bacteria were isolated, all were opportunistic pathogens, among which 29 strains were Ralstonia mannitolilytica and 10 strains were Pseudomonas aeruginosa. Conclusion Medical institutions should pay more attention to the microbial contamination of water for endoscopic diagnosis and treatment, water injection bottles have become a potential source of water contamination, in order to reduce potential risk of endoscopic diagnosis and treatment, continuous use of water injection bottles should not exceed one day.