精制抗眼镜王蛇毒血清的制备

 目的研制抗眼镜王蛇毒血清,以解决被眼镜王蛇咬伤后的救治问题。方法以眼镜王蛇毒为免疫原免疫马获得免疫血浆,经盐析制备IgG,IgG再经酶解、疏水柱纯化后得到F(ab′)₂活性片段,并以免疫扩散、ELISA测定其抗体特异性,以小鼠实验法测定其中和眼镜王蛇毒能力。结果得到的抗血清F(ab′)₂冻干品纯度达90%以上。结论研制出了特异性强、效价高的抗眼镜王蛇毒血清。

Study on Preparation of Purified Ophiophagus hannah Antivenin

OBJECTIVE To prepare the Ophiophagus hannah antivenin to cure the snakebite which was caused by the title snake.METHODS The IgG was firstly prepared by ammonium sulfate precipitation from horse plasma which was immunificated by Ophiophagus hannah venom,then subjected to pepsin-digesting and hydrophobic chromatography,the F(ab')₂ active fragment was ob- tained.The specificity of antivenin was traced through ELISA and the immunodiffusion,as well as protective test was performed on mice in vitro.RESULTS The purity of F(ab')₂ active fragment was over 90%.CONCLUSION The study led to the preparation of Ophiophagus hannah antivenin with high speciality and high potency.