多色流式细胞术对60例成人急性髓系白血病免疫分型的研究

目的探讨成人急性髓系白血病(AML)多色流式细胞术免疫分型的特点。方法采用CD45/SSC双参数散点图设门方法进行多色流式细胞术免疫表型分析。结果髓系抗原CD13和CD33在AML各亚型中均有很高的表达,阳性率分别为98.3%和85.0%。CD14和CD64在AML-M4及AML-M5患者中的表达率较高。CD14和CD64在AML-M4的表达率分别为60.0%和100.0%,在AML-M5的表达率分别为80.0%和100.0%,但CD14的特异性要高于CD64。AML患者CD34、CD117、HLA-DR的表达率分别为59.3%、84.3%和71.7%;AML-M3患者CD117表达率为76.9%,CD34及HLA-DR呈低表达。AML患者淋系抗原CD7和CD19阳性率分别为20.0%和3.3%。结论采用CD45/SSC双参数散点图设门方法,使成人AML免疫分型结果更可靠。CD13、CD33、CD14可作为AML免疫分型的髓系单抗。CD34及HLA-DR低表达为AML-M3的特征。成人AML淋系抗原表达以CD7较常见。

Research on Immunophenotyping with Muti-color Flow Cytometry in Sixty Adults with Acute Myeloid Leukemia

Objective To evaluate the charateristics of immunophenotyping in adults with acute myeloid leukemia（AML） using multi-color flow cytometry.Methods Immunophenotyping was performed with multi-color flow cytometry using CD₄₅ /SSC gating.Results The myeloid antigen of CD₁₃ and CD₃₃ was highly expressed in all patients with AML. The positive rate was 98.3 % and 85.0% respectively. The expression of CD₁₄ and CD₆₄ in patients with AML-M₄ and AML-M₅ were also high. The positive rate was 60.0%, 100.0% in AML-M₄ and 80.0%, 100.0% in AML-M₅ respectively. The expression of CD₃₄ 、CD₁₁₇ and HLA-DR in patients with AML was 59.3%, 84.3% and 71.7% respectively. CD₁₁₇ was expressed in 76.9% of patients with AML-M₃. Expression of CD₃₄ and HLA-DR was low in patients with AML-M₃. CD₇ and CD₁₉ expression in patients with AML was 20.0% and 3.3% respectively.Conclusion The immunophenotyping results of adults with AML were more reliable with gating of CD₄₅ /SSC. Monoclonal antibodies of CD₁₃, CD₃₃ and CD₁₄ can be used to detect the immunophenotyping in AML. The expression of CD₃₄ and HLA-DR was low in patients with AML-M₃. The expression of CD₇ lymphoid-associated antigens is relatively common in adults with AML.