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| 抗-HIV不同检测方法的结果比较 | |
| 引用本文： | 胡京辉,王瑞,孙莉. 抗-HIV不同检测方法的结果比较[J]. 检验医学与临床, 2008, 5(11): 656-657 |
| 作者姓名： | 胡京辉 王瑞 孙莉 |
| 作者单位： | 北京市血液中心检验科,100088;北京市血液中心检验科,100088;北京市血液中心检验科,100088 |
| 摘 要： | 目的探讨不同检测方法、不同检测试剂对献血者人类免疫缺陷病毒抗体（抗-HIV）检测结果的影响。方法采用不同试剂使用酶联免疫吸附试验（ELISA）对献血者血浆标本进行抗-HIV分析，呈反应性血浆标本采用免疫学蛋白印迹法进行确证分析。结果ELISA再次检测抗-HIV阳性率显著低于初次检测阳性率，差异有统计学意义（P〈0．01），确证试验阳性率显著低于初次检测和再次检测的阳性率，差异有统计学意义（P〈0．01）。结论采用不同试剂检测抗-HIV对于保证血液安全是非常必要的。 |
| 关 键 词： | 艾滋病 人类免疫缺陷病毒抗体 酶联免疫吸附试验 |
| 文章编号： | 1672-9455(2008)11-656-02 |
| 修稿时间： | 2008-02-18 |
Result comparison of different anti-HIV antibody detection methods |
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| HU Jing-hui,WANG Rui,SUN Li. Result comparison of different anti-HIV antibody detection methods[J]. Laboratory Medicine and Clinic, 2008, 5(11): 656-657 | |
| Authors: | HU Jing-hui WANG Rui SUN Li |
| Affiliation: | ( Department of Clinical Laboratory, Beijing Red Cross Blood Center, Beijing 100088, China) |
| Abstract: | Objective To investigate the impact of different detection methods and reagents on testing result of anti-HIV antibody in blood donors. Methods Anti-HIV antiboby was detected with ELISA using different reagents in blood donors, and positive sera were examined by Western blotting confirmatory test. Results The positive rate of anti-HIV by confirmatory test was significant lowed than that by ELISA（P〈0.01）. Conclusion It is very important to adopt different reagents in testing anti-HIV in blood donors for blood safety. |
| Keywords: | acquired immune deficiency syndrome （AIDS） anti-human immunodeficiency virus （HIV） antibody enzyme linked immunosorbent assay （ELISA） |
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